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Low Cost and Rapid DNA-Based Biometric Device

Award Information
Agency: Department of Defense
Branch: Special Operations Command
Contract: H92222-11-C-0030
Agency Tracking Number: S2-0166
Amount: $3,381,403.00
Phase: Phase II
Program: SBIR
Solicitation Topic Code: H-SB09.1-001
Solicitation Number: 2009.1
Timeline
Solicitation Year: 2009
Award Year: 2011
Award Start Date (Proposal Award Date): 2011-08-12
Award End Date (Contract End Date): N/A
Small Business Information
830 Winter Street, Waltham, MA, -
DUNS: 093286834
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Richard Selden
 Executive Chairman
 (781) 810-4904
 richard.selden@netbio.com
Business Contact
 Susan O'Keefe
Title: VP of Finance and Operati
Phone: (781) 916-9052
Email: susan.okeefe@netbio.com
Research Institution
 Stub
Abstract
Sensitive site exploitation activities are focused on collecting forensic evidence left behind by persons involved in terrorist or other illegal activity, and the biological evidence recovered is often referred to as"touch DNA samples". Touch samples include fingerprints, skin cells found on clothing (e.g. a shirt collar), and oral epithelial cells found on the opening of a soda can or the rim of a drinking glass. The quantity of DNA that is recovered from touch DNA samples is highly variable, ranging from less than 6 picograms (pg)the amount of DNA in a single human cell, to 100 ng. The proposed Rapid DNA-Based Biometric Device will include subsystems for sample preparation (cell lysis and purification), multiplex PCR amplification using NDIS/CODIS-compatible STR primer sets, electrophoretic length separation of PCR products, and laser-induced fluorescence detection of the products to yield electrophoretic traces. These subsystems will be based on microfluidic instrumentation and biochips previously developed and tested by NetBio for the evaluation of forensic samples containing much larger quantities of DNA than present within touch samples. Accordingly, the focus of the proposed work is to adapt the existing technology to the generation of STR profiles from touch samples.

* Information listed above is at the time of submission. *

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