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Porous Membrane Electrode for Quantitative Detection of Toxins

Award Information
Agency: Department of Commerce
Branch: N/A
Contract: WC-133R-12-CN-0071
Agency Tracking Number: 12-116
Amount: $94,206.78
Phase: Phase I
Program: SBIR
Solicitation Topic Code: 8.1.6N
Solicitation Number: 1
Solicitation Year: 2012
Award Year: 2012
Award Start Date (Proposal Award Date): 2012-08-01
Award End Date (Contract End Date): 2013-01-31
Small Business Information
4802 Glendarion Drive
Durham, NC 27713-8025
United States
DUNS: 033608972
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: Unavailable
Principal Investigator
 Thomas Stewart
 (866) 861-5836
Business Contact
Research Institution

There is a need for a simple, portable, quantitative method to monitor the presence of Harmful Algal Blooms. Electrochemical detection offers some advantages for analysis, but traditional sensors are not practical for routine field use. Adapting colorimetric immunoassays on porous, flow-through electrodes can provide a means for rapid, low-cost, portable quantitative environmental analysis.

This concept will be demonstrated in Phase I with a novel conductive, porous-membrane immunosensor for domoic acid. Antibodies will be attached to the electrode surface for capture of domoic acid and domoic acid-horseradish peroxidase conjugate. The immunoassay response will be measure using hydrogen peroxide and 3,3,5,5-tetramethylbenzidine as the enzyme comparison of the sample signal to a calibration curve. The porous design of the electrode allows assays to be performed quickly, simply and accurately.

Phase I will focus on:
1. Production of conductive, porous membranes with a layer of antibody attached to the conductive surface.
2. Demonstration of electrochemical detection of domoic acid using these membranes.
Phase II will incorporate the prototype membrane electrode and assay into a portable, low-cost, automated potentiostat that will be designed.

* Information listed above is at the time of submission. *

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