Applications and Methods for Continuous Monitoring of Physiological Chemistry

Award Information
Agency: Department of Defense
Branch: Defense Advanced Research Projects Agency
Contract: W31P4Q-12-C-0218
Agency Tracking Number: D12A-003-0016
Amount: $99,350.00
Phase: Phase I
Program: STTR
Awards Year: 2012
Solicitation Year: 2012
Solicitation Topic Code: SB12A-003
Solicitation Number: 2012.A
Small Business Information
Pinnacle Technology, Inc.
2721 Oregon Street, Lawrence, KS, -
DUNS: 96-172161
HUBZone Owned: N
Woman Owned: Y
Socially and Economically Disadvantaged: N
Principal Investigator
 Peter Petillo
 Chief Science Officer
 (785) 832-8866
 alchmist@pinnaclet.com
Business Contact
 Donna Johnson
Title: President&CEO
Phone: (785) 832-8866
Email: donnaj@pinnaclet.com
Research Institution
 The University of Kansas
 Joanne Altieri
 KU Center for Research
Youngberg Hall
Lawrence, KS, 66045-7563
 (785) 864-3444
 Nonprofit college or university
Abstract
With the notable exception of glucose, most metabolic biomarkers cannot be accurately, and practically measured over long periods, in vivo and in real-time. A primary reason is stable, selective enzymes suitable for biosensors are not available. Biosensors could revolutionize acute and long-term monitoring in the development, clinical, first responder and battlefield arenas. Pinnacle has developed intellectual property (patent PCT/US11/51193) that provides a general solution for the use of cofactors to enhance the biosensors sensitivity. We have also discovered that fusion proteins can dramatically enhance the stability of oxidases without compromising catalytic activity. Using high throughput screening in a cell-free system, directed evolution and large scale protein production in E.coli, we propose to develop a platform methodology for the optimization of existing (e.g. histamine), and the creation of new, (e.g.cortisol) oxidases for biosensors. Pinnacle Technology manufactures, sells and warranties biosensors for the detection of glucose, glutamate, lactate, and alcohol in animals. With the Richter and Gao laboratories at the University of Kansas we have successfully cloned, expressed, purified and characterized oxidases for biosensors. The enzymes produced will be available for direct sale, or as biosensors. World-wide total biosensor sales are expected to reach $14 billion by 2016.

* information listed above is at the time of submission.

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