Applications and Methods for Continuous Monitoring of Physiological Chemistry

Award Information
Agency:
Department of Defense
Branch
Defense Advanced Research Projects Agency
Amount:
$99,350.00
Award Year:
2012
Program:
STTR
Phase:
Phase I
Contract:
W31P4Q-12-C-0218
Award Id:
n/a
Agency Tracking Number:
D12A-003-0016
Solicitation Year:
2012
Solicitation Topic Code:
SB12A-003
Solicitation Number:
2012.A
Small Business Information
2721 Oregon Street, Lawrence, KS, -
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
Y
Duns:
96-172161
Principal Investigator:
PeterPetillo
Chief Science Officer
(785) 832-8866
alchmist@pinnaclet.com
Business Contact:
DonnaJohnson
President&CEO
(785) 832-8866
donnaj@pinnaclet.com
Research Institute:
The University of Kansas
Joanne Altieri
KU Center for Research
Youngberg Hall
Lawrence, KS, 66045-7563
(785) 864-3444

Abstract
With the notable exception of glucose, most metabolic biomarkers cannot be accurately, and practically measured over long periods, in vivo and in real-time. A primary reason is stable, selective enzymes suitable for biosensors are not available. Biosensors could revolutionize acute and long-term monitoring in the development, clinical, first responder and battlefield arenas. Pinnacle has developed intellectual property (patent PCT/US11/51193) that provides a general solution for the use of cofactors to enhance the biosensors sensitivity. We have also discovered that fusion proteins can dramatically enhance the stability of oxidases without compromising catalytic activity. Using high throughput screening in a cell-free system, directed evolution and large scale protein production in E.coli, we propose to develop a platform methodology for the optimization of existing (e.g. histamine), and the creation of new, (e.g.cortisol) oxidases for biosensors. Pinnacle Technology manufactures, sells and warranties biosensors for the detection of glucose, glutamate, lactate, and alcohol in animals. With the Richter and Gao laboratories at the University of Kansas we have successfully cloned, expressed, purified and characterized oxidases for biosensors. The enzymes produced will be available for direct sale, or as biosensors. World-wide total biosensor sales are expected to reach $14 billion by 2016.

* information listed above is at the time of submission.

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