Intracellular Detection of Small Molecules in Live Cells

Award Information
Agency: Department of Defense
Branch: Air Force
Contract: FA8650-12-M-5125
Agency Tracking Number: F11B-T09-0279
Amount: $100,000.00
Phase: Phase I
Program: STTR
Awards Year: 2012
Solicitation Year: 2011
Solicitation Topic Code: AF11-BT09
Solicitation Number: 2011.B
Small Business Information
P.O. Box 100, Ithaca, NY, -
DUNS: 022552900
HUBZone Owned: Y
Woman Owned: N
Socially and Economically Disadvantaged: Y
Principal Investigator
 Joel Tabb
 Principal Scientist
 (607) 272-0002
Business Contact
 Noe Salazar
Title: President
Phone: (512) 373-8601
Research Institution
 Cornell University
 Linda Brainard
 12 Day Hall
Ithaca, NY, 14853-4853
 (607) 255-7123
 Nonprofit college or university
ABSTRACT: The extreme toxicity of organophosphate (OP) chemical warfare agents (CWA) is due to their specific, rapid, and irreversible reaction with cholinesterase enzymes. A significant portion of this reactive nature of OP agents is directly due to the unique metabolically active environment found in and around cells. The goal of this proposal is to harness this unique intracellular environment to create a new class of highly sensitive, robust, and field portable sensor for rapidly detecting military personnel exposure to OP CWAs. In its final configuration, the proposed nanoparticles could be delivered as intradermal tattoos, allowing for rapid and personalized detection of potentially toxic OP agents. In Phase I, Agave BioSystems, in collaboration with Dr. Carl Batt of Cornell University, proposes to develop a warfare agent (CWA) detection system by incorporating fluorescent dyes demonstrated to sense CWAs into biocompatible nanoparticles that will serve as the support for the dye and the site of the response. The ability of these dye-impregnated nanoparticles to detect the presence of organophosphate CWAs will be tested in human cells. The goal is to create a system that utilizes human cellular chemistry to aid in the detection of potential CWA exposure. BENEFIT: In addition to detecting organophosphates, these types of nanoparticle sensors will have applications beyond CWAs since the nanoparticles are capable of supporting dyes capable of sensing myriad of other toxicants biomolecules. The potential diagnostic markets for this approach extend to VOC and other toxic industrial chemical detection in homeland security, law enforcement and industrial hygiene settings. The nanoparticles-based sensors could also be adapted for the detection of lactate, glucose, hydration, and corticosteroids levels.

* Information listed above is at the time of submission. *

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