A Rapid Coliform Detector

Award Information
Agency:
National Aeronautics and Space Administration
Branch
n/a
Amount:
$125,000.00
Award Year:
2012
Program:
SBIR
Phase:
Phase I
Contract:
NNX12CE71P
Award Id:
n/a
Agency Tracking Number:
114891
Solicitation Year:
2011
Solicitation Topic Code:
X3.03
Solicitation Number:
n/a
Small Business Information
WI, Madison, WI, 53717-1961
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
196894869
Principal Investigator:
Ross Remiker
Principal Investigator
(608) 229-2746
remikerr@orbitec.com
Business Contact:
Thomas Crabb
Business Official
(608) 827-5000
proposal@orbitec.com
Research Institution:
Stub




Abstract
ORBITEC, in collaboration with Lucigen, proposes a rapid genetic detector for spaceflight water systems to enable real-time detection of E. coli with minimal consumables and crew time. The Rapid Coliform Detector (RCD) amplifies the genetic material in a liquid sample to allow near real-time identification of specific genetic sequences, in this case, that of E. coli. This easy-to-use device incorporates a patented polymerase enzyme that enables rapid RNA amplification by reagents with superior long-term shelf life and thermal stability. A color change indicator will show the presence or absence of coliform bacteria in the water within 30 minutes. The results of the Phase 1 will be test data from prototype test kits and chemical reagents for rapid coliform detection which brings the RCD to TRL 4. The anticipated results of the Phase 2 are a flight-like prototype of the complete test kit and reaction chamber, performance test results at 1g, and reduced gravity operational test results, which bring the technology to TRL 6.

* information listed above is at the time of submission.

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