A Rapid Coliform Detector

Award Information
Agency: National Aeronautics and Space Administration
Branch: N/A
Contract: NNX12CE71P
Agency Tracking Number: 114891
Amount: $125,000.00
Phase: Phase I
Program: SBIR
Awards Year: 2012
Solicitation Year: 2011
Solicitation Topic Code: X3.03
Solicitation Number: N/A
Small Business Information
Orbital Technologies Corporation
WI, Madison, WI, 53717-1961
DUNS: 196894869
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Ross Remiker
 Principal Investigator
 (608) 229-2746
Business Contact
 Thomas Crabb
Title: Business Official
Phone: (608) 827-5000
Email: proposal@orbitec.com
Research Institution
ORBITEC, in collaboration with Lucigen, proposes a rapid genetic detector for spaceflight water systems to enable real-time detection of E. coli with minimal consumables and crew time. The Rapid Coliform Detector (RCD) amplifies the genetic material in a liquid sample to allow near real-time identification of specific genetic sequences, in this case, that of E. coli. This easy-to-use device incorporates a patented polymerase enzyme that enables rapid RNA amplification by reagents with superior long-term shelf life and thermal stability. A color change indicator will show the presence or absence of coliform bacteria in the water within 30 minutes. The results of the Phase 1 will be test data from prototype test kits and chemical reagents for rapid coliform detection which brings the RCD to TRL 4. The anticipated results of the Phase 2 are a flight-like prototype of the complete test kit and reaction chamber, performance test results at 1g, and reduced gravity operational test results, which bring the technology to TRL 6.

* information listed above is at the time of submission.

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