FINDING NOVEL REAGENTS BY BIOINFORMATICS AND BIOCHEMISTRY

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$874,892.00
Award Year:
2012
Program:
SBIR
Phase:
Phase II
Contract:
4R44GM100560-02
Agency Tracking Number:
R44GM100560
Solicitation Year:
2012
Solicitation Topic Code:
NIGMS
Solicitation Number:
PA11-096
Small Business Information
NEW ENGLAND BIOLABS, INC.
240 County Road, Ipswich, MA, -
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
66605403
Principal Investigator:
RICHARD ROBERTS
(978) 380-7405
roberts@neb.com
Business Contact:
BRIAN TINGER
(978) 380-7485
tinger@neb.com
Research Institution:
Stub




Abstract
DESCRIPTION (provided by applicant): This project aims to find novel restriction enzymes that will be useful in many aspects of molecular biology research. One particular focus will be on restriction endonucleases that are able to recognize modified DNA, especially DNA containing 5-methylcytosine and 5- hydroxymethycytosine since such enzymes have many potential applications in epigenetics research. Enzymes recognizing these modified bases are known to occur in bacterial and archaea strains and in the past,many have been found by random biochemical screening. In the proposed project, the discovery of potential new enzymes will use bioinformatics techniques and take advantage of the large number of currently sequenced prokaryotic genomes, both completely sequenced genome and shotgun sequences. The project will identify good candidates and then use biochemical methods to characterize them. In addition to commercializing any useful enzymes that are found from this screen, the information gained about the biochemical function of the restriction enzymes discovered will be invaluable for genome annotation and will have practical implications for researchers working with pathogenic organisms. The latter will result from our efforts to characterize the restriction-modification systems in pathogens and identify those systems which need either to be knocked out or circumvented by prior modification prior to transformation. Thus, the results of this research will have immediate implications for the fields of epigenetics,pathogenesis and genome annotation.

* information listed above is at the time of submission.

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