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Portable Lab-on-a-chip Flow Cytometer: Prototype and Application Development

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 9R44GM103677-02
Agency Tracking Number: R44GM103677
Amount: $972,199.00
Phase: Phase II
Program: SBIR
Solicitation Topic Code: NIGMS
Solicitation Number: PA11-096
Solicitation Year: 2012
Award Year: 2012
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
7770 REGENTS RD #113390
United States
DUNS: 832751098
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 (951) 966-4963
Business Contact
Phone: (858) 699-5230
Research Institution

DESCRIPTION (provided by applicant): Portable Lab-on-a-chip Flow Cytometer: Prototype and Application Development NanoSort RESEARCH and RELATED Other Project Information 7. PROJECT SUMMARY The lab-on-a-chip NanoSort device proposed here advances the achievements of the Phase I Beta Prototype project. This device will be appropriate for commercial success by improving access to and reducing the cost and complexity of sorting flow cytometry. Furthermore, it will expand the applications that this device can serve, including translational and clinical point-of-care flow cytometry applications for diagnostics, prognosis, and personalized medicine. Having completed key milestones in the Phase I program, such as mas- producible injection molded chips, advanced signal detection and processing and key performance metrics, we propose to extend the capabilities to compete with best-in-class devices. Our Aims for this project are to create a device (NanoSort-1 or NS-1) that will have improved performance parameters of 6-color detection with a single photomultiplier tube (PMT), 4-way sorting using low shear stress piezoelectric (PZT) actuators, and a robust digital signal processing algorithm (Color-Space-Time (CoST) implementation that allows 10,000 events per second detection and sorting. In addition to these innovations, we will construct the device using current good manufacturing processes (cGMP) to assure that this device will be UL and CE certified and scalable to 10,000 units per year. We will validate this device with consultation from established researchers at UCSD and device manufactures (Becton-Dickinson and Life Technologies). PUBLIC HEALTH RELEVANCE: Portable Lab-on-a-chip Flow Cytometer: Prototype and Application Development NanoSort RESEARCH and RELATED Other Project Information 8. PROJECT NARRATIVE Important techniques in biomedical research include the counting (flow cytometry) and separation (cell sorting) of cells or particles based on their innate properties, currently performed by very large (~800lbs) and expensive (~ 500,000) machines that require dedicated space and staff for their operation. We have invented and patented three techniques that enable the development of a sorting flow cytometer at a fraction of the cost and size of conventional instruments, using microfluidics to channel cells, piezoelectric actuators to sort cells, and a technique called Color-Space-Time coding to detect several colors with a single detector (as opposed to one detector per color in conventional instruments). Reductions in costs and complexity make this new nanosorting device accessible to any researcher, advancing biomedical discovery.

* Information listed above is at the time of submission. *

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