Engineering Photosynthetic Cyanobacteria to Produce the Energy-Dense Fuel Farnesene from Carbon Dioxide

Award Information
Agency:
Department of Agriculture
Branch
n/a
Amount:
$99,875.00
Award Year:
2012
Program:
SBIR
Phase:
Phase I
Contract:
2012-00437
Agency Tracking Number:
2012-00437
Solicitation Year:
2012
Solicitation Topic Code:
8.8
Solicitation Number:
USDA-NIFA-SBIR-003497
Small Business Information
DAST
815 MEDARY AVE STE 201, Brookings, SD, 57006-1303
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
830774456
Principal Investigator:
Tyler Remund
(605) 695-6631
tyler@southdakotainnovation.com
Business Contact:
Mark Luecke
President
(605) 366-3151
mark@southdakotainnovation.com
Research Institution:
Stub




Abstract
The goal of the project is to genetically engineer photosynthetic cyanobacteria that will synthesize farnesene from carbon dioxide. The work will then involve shutting down pathways that compete for carbon in an attempt to maximize the amount of farnesene that can be produced. Then we will do tolerance testing by culturing the cyanobacteria in varying concentrations of the end product(s) in order to determine the optimal culturing conditions. This work will develop an engineered cyanobacterium capable of converting CO2 into farnesene (C15H24), a long-chain hydrocarbon with an energy density of 47 MJ/Kg, heat of vaporization of 0.24 MJ/Kg, and octane of 89.7. The cells would excrete farnesene into the recirculating culture fluid, where it would be recovered by low-cost phase separation for use as an energy-dense, infrastructure compatible biofuel. We have already established the validity of this "cyanofactory" platform by engineering Anabaena to produce the ten-carbon alcohol linalool. Farnesene has a much higher energy density and thus would be a more desirable 3rd generation biofuel.

* information listed above is at the time of submission.

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