Microbial production of dicarboxylic acids

Award Information
Agency:
Department of Energy
Amount:
$988,214.00
Program:
SBIR
Contract:
DE-FG02-11ER90193
Solitcitation Year:
2012
Solicitation Number:
DE-FOA-0000676
Branch:
N/A
Award Year:
2012
Phase:
Phase II
Agency Tracking Number:
96120
Solicitation Topic Code:
02 c
Small Business Information
Lygos, Inc
77 Van Ness Ave., Ste 101, Mail Stop# 1953, San Francisco, CA, 94102-6042
Hubzone Owned:
N
Woman Owned:
N
Socially and Economically Disadvantaged:
N
Duns:
963901470
Principal Investigator
 Jeffrey Dietrich
 Dr.
 (281) 772-4475
 jadietrich@lygos.com
Business Contact
 Jeffrey Fortman
Title: Dr.
Phone: (612) 590-9639
Email: clem@lygos.com
Research Institution
 Stub
Abstract
Dicarboxylic acids are important industrial chemicals currently produced from petroleum using ecologically damaging manufacturing methods; furthermore, dicarboxylic acid prices are rising with increasing petroleum feedstock prices. Lygos is developing microbial hosts for the cost-competitive production of dicarboxylic acids from renewable feedstocks, including cellulosic hydrolysate. The combined Phase I/II approach is the development and use of a high-throughput dicarboxylic acid screen to assay for improved dicarboxylic acid production from engineered microbes. In Phase I, we constructed and characterized three dicarboxylic acid screens applicable for short- to medium-chain microbially produced diacids (up to C7). The screens are based on microbial sensing of exogenous dicarboxylic acid and an associated increase in a colorimetric reporter output. The dicarboxylic acid screen will now be implemented as a high-throughput assay for improved dicarboxylic acid production in engineered microbes. Combinatorial libraries of engineered proteins and metabolic pathways will be tested for improvements in strain yield, titer, and productivity. Dicarboxylic acid production in the evolved strain will be also demonstrated using a cellulosic hydrolysate sugar source. Commercial Applications and Other Benefits: Dicarboxylic acids are high-value chemicals used in the manufacturing of commodity and specialty goods. Lygos evolved microbial production platform will enable the cost- competitive production of diacids from renewable feedstocks, providing both an economic and environmental benefit. A key aspect of the grant effort is the production of diacids using cellulose-derived sugars.

* information listed above is at the time of submission.

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