A Binary Probe-Based DNAzyme Cascade for Rapid Detection of MRSA/MSSA

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$168,547.00
Award Year:
2012
Program:
STTR
Phase:
Phase I
Contract:
1R41AI100468-01
Award Id:
n/a
Agency Tracking Number:
R41AI100468
Solicitation Year:
2012
Solicitation Topic Code:
NIAID
Solicitation Number:
PA11-097
Small Business Information
535 W Research Blvd., Suite 135, M/S 300, FAYETTEVILLE, AR, 72701-
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
125476825
Principal Investigator:
XIAOLI SU
(479) 571-2592
xiaoli.su@biodetection-instruments.com
Business Contact:
MARK KIDD
(479) 571-2592
mark.kidd@biodetection-instruments.com
Research Institute:
UNIVERSITY OF CENTRAL FLORIDA

UNIVERSITY OF CENTRAL FLORIDA
12201 Research Parkway, Ste 501
ORLANDO, FL, 32826-3246
() -
Nonprofit college or university
Abstract
DESCRIPTION (provided by applicant): A binary probe-based DNAzyme cascade for rapid detection of MRSA/MSSA Project Summary This STTR Phase I project will develop a proof-of-concept molecular test for rapid detection of Staphylococcus aureus (SA) and differentiation between methicillin-resistant SA (MRSA) and methicillin-susceptible SA (MSSA) in the absence of PCR amplification. Binary DNAzyme probes will be designed to be complementary to an MRSA-specific gene or to an SA-specific gene. In presence of a target gene, the binary probes will be assembled into a catalytically active DNAzyme and activate a DNAzyme cascade, providing a visual or colorimetric readout. The Phase I research will focus on designing the binary DNAzyme probes, constructing the DNAzymecascade, and demonstrating the technical feasibility for MRSA/MSSA screening. Compared to MSSA infections, MRSA infections have limited treatment options and result in significantly higher morbidity, mortality, and healthcare costs. Therefore, rapid and reliable detection of SA and differentiation between MRSA and MSSA will have significant clinical, economic, and societal impacts. If successfully developed, the proposed DNAzyme cascade approach will significantly improve the detection limit of current PCR-free molecular tests and have the potential to be developed into commercially viable, self-contained single-tube assays with minimum hands-on time and no requirement for sophisticated instrument. PUBLIC HEALTH RELEVANCE: Staphylococcus aureus, particularly MRSA, is a major cause of infections ranging from skin and soft tissue infections to invasive, life-threatening infections such as bacteremia, endocarditis, etc. The proposed PCR-free molecular test would address an urgent and unmet need for rapid, sensitive, and specific screening of MRSA and MSSA in an easy-to-use, cost- effective way, thereby allowing for timely and accurately treatment of MRSA/MSSA infections and helping reduce the associated healthcare costs.

* information listed above is at the time of submission.

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