Real Time Multidimensional Spectroscopic Assessment of Tissue Viability

Award Information
Agency: Department of Defense
Branch: Defense Health Program
Contract: W81XWH-12-C-0078
Agency Tracking Number: O112-H08-3019
Amount: $149,925.00
Phase: Phase I
Program: SBIR
Awards Year: 2012
Solicitation Year: 2011
Solicitation Topic Code: OSD11-H08
Solicitation Number: 2011.2
Small Business Information
4041 Forest Park Ave., St. Louis, MO, -
DUNS: 176142693
HUBZone Owned: Y
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Timothy Holmes
 Chief Executive Officer
 (314) 615-6921
Business Contact
 Maria Holmes
Title: Chief Financial Officer
Phone: (314) 615-6922
Research Institution
The objective of Phase I is the preliminary design, prototype and testing of a 3D spectral imaging system for the characterization and evaluation of viability of engineered tissue for treating war injuries. The purpose is in ensuring tissue viability, maturity, vascularity to know if a construct is ready for transplantation. The hardware will consist of a widefield microscope with 3D optical sectioning, lateral scanning for large constructs, and a spectrally programmable light source. Software algorithms will produce the spectral images, deconvolve them to eliminate out-of-focus blur, and align and seam adjacent 3D fields. For characterization and monitoring, algorithms will analyze the vascularization, cell proliferation, motility, spectra and other measures. The system will be for live imaging so it will use no dyes and will utilize endogenous contrast-producing optical characteristics of the construct. Testbed tissue-engineering applications include the development of (1), 3D scaffolds, (2), microvascular network formation in regenerative tissue, and (3), several clinically functional tissue types. Phase I will demonstrate the prototype to show that 3D spectral images can be produced with a testbed sample - most notably the scaffolds with microvascularization. Phase II will embed the proposed system within the study and development of these three construct types.

* Information listed above is at the time of submission. *

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