Use of ECIS Technology for Real-Time Non-Invasive Identification of Partially and/or Fully Differentiated Cells for Regenerative Medicine

Award Information
Agency: Department of Defense
Branch: Defense Health Program
Contract: W81XWH-12-C-0068
Agency Tracking Number: O112-H14-3143
Amount: $149,953.00
Phase: Phase I
Program: SBIR
Awards Year: 2012
Solicitation Year: 2011
Solicitation Topic Code: OSD11-H14
Solicitation Number: 2011.2
Small Business Information
DUNS: 612373964
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Gabriela Tudor
 Senior Scientist
 (412) 402-9925
Business Contact
 George Sing
Title: President&CEO
Phone: (917) 882-8001
Research Institution
The Electric cell-substrate impedance sensing (ECIS) technology provides an excellent miniaturized platform on which to design and execute non-invasive and label-free specialized biological assays to continuously monitor cellular parameters such as viability, adhesion, and proliferation in a high throughput way. It records electrical impedance of cells grown on microelectrodes integrated into the bottom of individual 8-wells or 96-wells arrays. It has been shown that throughout differentiation, the gradual reorganization of the cell layer can be accurately detected using the ECIS modeling of the complex impedance and its components. We propose to use the ECIS technology for real-time, label-free monitoring of stem cell differentiation by recording cell-specific behaviors critical to differentiation, in a high throughput format. We intend to reveal cell-identity maps which can be used to create cell-type specific signatures where each cell type can be associated with specific differentiation-induction response-curves. Overall, we will evaluate impedance signaling associated with changes in cell-cell contacts, modifications in cell-substrate interactions, proliferation, spreading and migration. We assume these early changes to be cell-type specific and the overall goal of this proposal is to demonstrate they can be annotated.

* information listed above is at the time of submission.

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