Feasibility Study to Explore NGS technologies for Pathogen Identification

Award Information
Agency: Department of Defense
Branch: Defense Health Program
Contract: W81XWH-12-C-0061
Agency Tracking Number: O112-H16-3101
Amount: $150,000.00
Phase: Phase I
Program: SBIR
Awards Year: 2012
Solicitation Year: 2011
Solicitation Topic Code: OSD11-H16
Solicitation Number: 2011.2
Small Business Information
8052 El Rio Street, Houston, TX, -
DUNS: 106700375
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 David Hertzog
 VP Business Development
 (713) 747-3380
 dhertzog@lasergen.com
Business Contact
 David Hertzog
Title: VP Business Development
Phone: (713) 747-3380
Email: dhertzog@lasergen.com
Research Institution
 Stub
Abstract
Pathogen detection involving microbial forensics is an emerging field that presents enormous challenges for both the scientific and legal communities. Microbial pathogens of humans represent a highly diverse set of organisms known to cause disease and death. Microbes have also developed a number of elaborate mechanisms for generating natural genetic diversity. One major goal of microbial forensics is to use this genetic diversity to identify the source of a pathogen used to commit a crime or war offensive. Recent advances in next-generation sequencing (NGS) technologies have the potential to significantly alter the technological approaches used in characterizing field samples. This Phase I SBIR grant application proposes three aims: (i) identify the most efficient NGS platform by sequencing E. coli MG1655 using six platforms, (ii), conduct mixing experiments using purified gram negative and gram positive bacteria using the platform selected in aim (i), and (iii) conduct mixing experiments described in aim (ii) in the presence of human blood to simulate animal wound models. The outcome of the Phase I study lays the foundation for a Phase II proposal to develop method/system that would be portable and useful as a point-of-care device for pathogen identification in the field.

* Information listed above is at the time of submission. *

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