Technologies for Treating Cartilage Tissue Loss Following Traumatic Injury

Award Information
Agency:
Department of Defense
Branch
n/a
Amount:
$729,999.00
Award Year:
2012
Program:
SBIR
Phase:
Phase II
Contract:
W81XWH-11-C-0071
Award Id:
n/a
Agency Tracking Number:
O2-1236
Solicitation Year:
2010
Solicitation Topic Code:
OSD10-H04
Solicitation Number:
2010.3
Small Business Information
800 E Leigh St Ste 51, Suite 51, Richmond, VA, 23219
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
808554526
Principal Investigator:
Anthony Nicolini
Research and Development
(404) 520-6226
anicolini@spheringenics.com
Business Contact:
L. Bost
President and CEO
(404) 385-2115
fbost@spheringenics.com
Research Institution:
Stub




Abstract
Phase I studies demonstrate that human adipose stem cells (ASCs) exhibit characteristics of chondrocytes when cultured in chondrogenic media (CM) and this is enhanced when they are first encapsulated in low viscosity, high mannuronate content alginate beads that are less than 200fYm in diameter containing 250 cells/bead. The microencapsulated cells express mRNAs for factors associated with chondrogenesis and secrete these factors into their media, suggesting that they will also do so in vivo. There are a number of questions remaining that must be addressed in order to move this technology to clinical application. Phase II research will demonstrate whether CM-treated microencapsulated ASCs will enhance cartilage repair and regeneration of craniofacial chondral defects in vivo. We propose to optimize processing conditions for chondrogenic growth factor production by determining if shorter CM-treatments are sufficient and if chondrogenic growth factor production varies among donors; identify the best processing protocol using a small rodent screening model by determining if CM-pretreatment time alters chondrogenic potential of microencapsulated ASCs in vivo and if donor variability is a factor; and test the ability of CM-treated microencapsulated ASCs will repair and regenerate craniofacial cartilage by assessing effectiveness in rabbit critical size auricular and nasal defects.

* information listed above is at the time of submission.

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