Deployable Pan-flavivirus and Pan-alphavirus Assays for Screening Pools of Medically Relevant Arthropod

Award Information
Agency: Department of Defense
Branch: Army
Contract: W81XWH-11-C-0046
Agency Tracking Number: A2-4911
Amount: $728,157.00
Phase: Phase II
Program: SBIR
Awards Year: 2012
Solicitation Year: 2010
Solicitation Topic Code: A10-158
Solicitation Number: 2010.3
Small Business Information
18916 North Creek Parkway, Suite 115, Bothell, WA, -
DUNS: 963449645
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 John Cooper
 Principal Scientist
 (425) 609-3079
Business Contact
 Brooke Anderson
Title: Chief Operating Officer
Phone: (425) 609-0923
Research Institution
CustomArray produces a field-deployable electrochemical microarray platform ideally suited for the multiplexed detection of pathogenic organisms. In this Phase II proposal, we will continue our Phase I effort to build an automated arthropod-borne virus detection system. The Phase I assay work-flow included the following three steps: (1) Extract RNA from Mosquitoes; (2) Amplify and label viral RNA loci; and (3) Detect amplification product on microarray. The results of the assay were analyzed, using software we created, to make a pathogen call. We will use this prototype detection assay as a launching pad for developing the instrumentation necessary to automate the assay and create a truly field-deployable system. In this proposal, we outline our strategy for this automation. We will (1) automate the sample prep system to extract and purify the RNA, requiring only an input of mosquitoes; (2) create an amplification system which will enable multiplexed amplification of all medically relevant arboviruses; and (3) automate the hybridization and electrochemical detection for hands free processing. We envision integrating the automated sample prep/amplification system into one disposable cartridge which will insert into a device for processing and have an output of hybridization ready material appropriate interrogation using the developed hybridizer/reader.

* Information listed above is at the time of submission. *

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