Microscale Tip Device for Rapid Concentration and Storage of PCR-ready DNA from B

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$150,000.00
Award Year:
2012
Program:
SBIR
Phase:
Phase I
Contract:
1R43GM099347-01A1
Agency Tracking Number:
R43GM099347
Solicitation Year:
2012
Solicitation Topic Code:
NIGMS
Solicitation Number:
PA11-096
Small Business Information
NANOFACTURE, INC.
16301 NE 8th St Ste 110, Bellevue, WA, 98008
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
620837299
Principal Investigator:
KYONG-HOON LEE
(425) 269-6051
hoonlee@nano-facture.com
Business Contact:
JIYOUNG KANG
(425) 830-0530
j.kang@nano-facture.com
Research Institution:
Stub




Abstract
DESCRIPTION (provided by applicant): The current SBIR Phase I proposal addresses a need for the rapid and simple sample preparation of genomic DNA from human saliva and blood through a novel technology: microscale tip enrichment probes that employ electric fields and capillary action. With the action of a microtip-installed device, DNA contained in a given saliva sample can be selectively captured without centrifugation on the proposed microtip, and stored as a dried form for long- term preservation and delivery. The preserved DNA on microtips is released into a desired medium for PCR analysis without additional purification steps. Therefore, this innovative technology enables the one-step concentration of DNA from human saliva and blood as a purified, durable form. This application describes a feasibility study of the proposed method and system to explore and optimize the protocol of centrifuge-free preparation of PCR-ready DNA. PUBLIC HEALTH RELEVANCE: This proposal aims to conduct a feasibilitystudy for a portable device capable of concentrating and purifying human genomic DNA from saliva without centrifuge. The success of the project would provide a convincing rationale and form a technical basis for the proposed methodology for rapid and simple preparation of PCR-ready DNA from various sample specimens with a high throughput at a low cost.

* information listed above is at the time of submission.

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