A low-cost instrument for nucleic acid isolation.

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R43GM103053-01
Agency Tracking Number: R43GM103053
Amount: $280,784.00
Phase: Phase I
Program: SBIR
Awards Year: 2012
Solicitation Year: 2012
Solicitation Topic Code: NIGMS
Solicitation Number: PA11-096
Small Business Information
400 Sagner Avenue, Suite 300, FREDERICK, MD, 21701-6082
DUNS: 154704444
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 CHRISTOPHER COONEY
 (301) 698-0101
 cooney@akonni.com
Business Contact
 CHRISTOPHER COONEY
Phone: (301) 698-0101
Email: cooney@akonni.com
Research Institution
 Stub
Abstract
DESCRIPTION (provided by applicant): Sample preparation frequently presents a bottleneck for clinical molecular biology workflows. Whereas there are many readout detection modalities, there are only a handful of automated sample preparation workflow strategies. Existing instruments that are built around these sample preparation strategies and chemistries range in cost from 17- 150k, and yet they still do not include an integrated method of lysing difficult-to-disrupt organisms such as gram-positive bacteria or the acid fast bacilli (i.e., Mycobacterium). To address these limitations, we propose to integrate Akonni's sample preparation technologies into a low-cost automated instrument that can process a variety of sample types such as blood, nasopharyngeal swabs, and nasopharyngeal aspirate with a simple-to-use consumable kit. PUBLIC HEALTH RELEVANCE: Molecular diagnostics, which are tests that analyze the DNA and/or RNA in a sample, are becoming increasingly prevalent among clinical laboratory testmethods. An important, yet challenging, procedure, required of these tests, is to remove the DNA/RNA from the sample and wash away the impurities. We seek to develop a low-cost automated instrument to address this challenge

* Information listed above is at the time of submission. *

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