An automated on-site detection system for viral pathogens of biomedical laborator

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R43OD012402-01
Agency Tracking Number: R43OD012402
Amount: $156,371.00
Phase: Phase I
Program: SBIR
Awards Year: 2012
Solicitation Year: 2012
Solicitation Topic Code: OD
Solicitation Number: PA11-340
Small Business Information
DUNS: 805888901
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 (479) 595-0320
Business Contact
Phone: (479) 595-0320
Research Institution
DESCRIPTION (provided by applicant): This SBIR project is a collaborative effort between AC Diagnostics, Inc. and the University of Pennsylvania to develop and demonstrate a novel diagnostic system for automated on-site detection of viral pathogens in biomedical laboratory animals. Microbial infections and outbreaks threaten the survival or utility of biomedical animal models used in current and future research. It is therefore crucial to accurately and timely identify current and emerging pathogens in labanimal populations and facilities to assure pathogen-free biological resources. Current diagnostic methods for the animal pathogens are time-consuming and expensive, and are therefore mainly limited to large research or diagnostic laboratories. There is nocommercial product available for routine applications. Consequently, automated, simple-to- operate technologies as standardized or user configurable commercial products are urgently needed for rapid, reliable and cost-effective detection and identification of the pathogenic microbes in laboratory animal populations and settings. The technology proposed in this project integrates automated viral RNA isolation from raw samples and real-time nucleic acid sequence-based amplification (NASBA) detection into asensitive, reliable and low-cost (lt 10) diagnostic system. We anticipate a limit of detection of less than 10 viruses, and test times within 60 minutes. The primary specific aims of Phase I are to develop a prototype assay system and demonstrate its feasibility for detecting animal viruses in blood. The ultimate goal of this project is to develop an on-site assay device which is consisted of single-use (disposable) self-contained credit-card sized assay cassette and a fully- automated portable detector for completely automated detection of laboratory animal viruses. The final product will be fully developed and validated in the Phase II of this project. The successful completion of this project will significantly advance animal virus detection, and provide a simple on-site assay device with minimum operator attention or skill requirement. The same technology can also be used as a platform for other pathogens such as viruses in nonhuman primates and bacterial pathogens, and as a part of standardized operation procedures in daily laboratory animal care. 1 PUBLIC HEALTH RELEVANCE: The successful completion of this project will significantly advance the technique of virus detection, and provide a general platform for the detection of other animal pathogens such as viruses in nonhuman primates and bacterial pathogens. This novel device will have widespread applicability as a standardized and streamlined commercial product and procedure for rapid, reliable and cost-effective detection and identificationof the microbes in laboratory animal cares. Therefore it will meet the requirements for establishing pathogen-free biological resources for current and future biomedical research needs.

* information listed above is at the time of submission.

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government