Development of Blood Donor Screening for Babesiosis

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$149,437.00
Award Year:
2012
Program:
SBIR
Phase:
Phase I
Contract:
200-2012-M-51644
Award Id:
n/a
Agency Tracking Number:
HHS2002012M51644
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Seattle, WA, 98104-
Hubzone Owned:
N
Minority Owned:
Y
Woman Owned:
Y
Duns:
004006628
Principal Investigator:
RaymondHoughton
raymond@inbios.com
Business Contact:
RaymondHoughton
raymond@inbios.com
Research Institute:
n/a
Abstract
Human Babesiosis is primarily caused by Babesia microti an intraerythrocytic parasite with some cases on the US west coast associated with Babesia duncani (WA-1). Typically tick bites are the route of transmission. However, both organisms have been associated with transfusion related infections and deaths in immunocompromized recipients. All indications are that the number of cases is on the rise and endemic regions might be expanding both in the US and Europe. Current diagnosis relies on blood smear, lmmunofluorescence (IFA), PCR and confirmation with Western blot. To date there is no FDA approved test for screening the blood supply but the FDA indicated the need for regional screening of blood supplies in July 201 O. Previously we have reported on a Babesia microti specific peptide ELISA that was able to detect a significant number of blood donor sera positive by IFA or western blot. The goal of this proposal is to identify additional immunodominant epitopes from a panel of proprietary recombinant proteins for B. microti (and later 8. duncani) previously selected using serological expression cloning. In addition, bioinformatics will be used to identify immunodominant tandem repeat sequences. These potential antigens will then be used in development of a sensitive ELISA and also potentially provide reagents for use on other blood testing instrumentation. To achieve these goals we have assembled a group of experts in recombinant protein design, ensured access to Babesia microti samples, recombinants and testing of prototypes at the Red Cross

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government