An automated, high throughput, resin-free device for large scale protein purification

Award Information
Agency: Department of Defense
Branch: Office for Chemical and Biological Defense
Contract: W911NF-13-C-0034
Agency Tracking Number: C122-102-0012
Amount: $99,863.00
Phase: Phase I
Program: SBIR
Awards Year: 2013
Solicitation Year: 2012
Solicitation Topic Code: CBD12-102
Solicitation Number: 2012.2
Small Business Information
CFD Research Corporation
215 Wynn Dr., 5th Floor, Huntsville, AL, -
DUNS: 185169620
HUBZone Owned: N
Woman Owned: Y
Socially and Economically Disadvantaged: N
Principal Investigator
 Hongjun Song
 Research Engineer
 (256) 327-5294
 proposals-contracts@cfdrc.com
Business Contact
 Deborah Phipps
Title: Contracts Manager
Phone: (256) 726-4884
Email: dap@cfdrc.com
Research Institution
 Stub
Abstract
Protein manufacturing is of paramount importance to chemical and biological defense applications. High-throughput protein purification is a critical need for rapidly scaling the target protein product. Existing purification methods primarly rely on synthetic-resin based chromatography, which is time-consuming, labor-intensive, expensive, and consequently, ill-suited for developing rapid countermeasures to chemical/biological threat. To overcome these limitations, we propose to develop and demonstrate a novel high-throughput, resin-free technology for automated protein recovery and purification. Our technology based on novel multi-scale electrokinetics for protein purification enables order-of-magnitude improvement in investment and operating cost as well as human labor. In Phase I, we will design, fabricate, characterize and demonstrate a pilot-scale prototype to establish proof-of-principle of the proposed technology. The designs of the purification device will be optimized using high-fidelity simulation tools. The fabricated device will be tested and demonstrated in our well-equipped bioengineering laboratories. In Phase II, a full-scale purification system with additional design refinements will be developed for enhanced performance and manufacturability. The purification module will be integrated with COTS technologies for automated operation. The Phase II prototype will be demonstrated for continuous, long-term purification of target proteins from composite sample matrix. The final product will be fully automated, high throughput and low cost system for rapid purification of desired proteins

* information listed above is at the time of submission.

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