An automated, high throughput, resin-free device for large scale protein purification

Award Information
Agency:
Department of Defense
Branch
n/a
Amount:
$99,863.00
Award Year:
2013
Program:
SBIR
Phase:
Phase I
Contract:
W911NF-13-C-0034
Award Id:
n/a
Agency Tracking Number:
C122-102-0012
Solicitation Year:
2012
Solicitation Topic Code:
CBD12-102
Solicitation Number:
2012.2
Small Business Information
215 Wynn Dr., 5th Floor, Huntsville, AL, -
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
Y
Duns:
185169620
Principal Investigator:
Hongjun Song
Research Engineer
(256) 327-5294
proposals-contracts@cfdrc.com
Business Contact:
Deborah Phipps
Contracts Manager
(256) 726-4884
dap@cfdrc.com
Research Institution:
Stub




Abstract
Protein manufacturing is of paramount importance to chemical and biological defense applications. High-throughput protein purification is a critical need for rapidly scaling the target protein product. Existing purification methods primarly rely on synthetic-resin based chromatography, which is time-consuming, labor-intensive, expensive, and consequently, ill-suited for developing rapid countermeasures to chemical/biological threat. To overcome these limitations, we propose to develop and demonstrate a novel high-throughput, resin-free technology for automated protein recovery and purification. Our technology based on novel multi-scale electrokinetics for protein purification enables order-of-magnitude improvement in investment and operating cost as well as human labor. In Phase I, we will design, fabricate, characterize and demonstrate a pilot-scale prototype to establish proof-of-principle of the proposed technology. The designs of the purification device will be optimized using high-fidelity simulation tools. The fabricated device will be tested and demonstrated in our well-equipped bioengineering laboratories. In Phase II, a full-scale purification system with additional design refinements will be developed for enhanced performance and manufacturability. The purification module will be integrated with COTS technologies for automated operation. The Phase II prototype will be demonstrated for continuous, long-term purification of target proteins from composite sample matrix. The final product will be fully automated, high throughput and low cost system for rapid purification of desired proteins

* information listed above is at the time of submission.

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