Rapid Fluorescent Assays for Coliforms in Shipboard Wastewater

Award Information
Agency: Department of Defense
Branch: Navy
Contract: N00024-13-P-4541
Agency Tracking Number: N121-053-0957
Amount: $79,998.00
Phase: Phase I
Program: SBIR
Awards Year: 2013
Solicitation Year: 2012
Solicitation Topic Code: N121-053
Solicitation Number: 2012.1
Small Business Information
1 Riverside Circle, Suite 400, Roanoke, VA, -
DUNS: 627132913
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Michael Danilich
 Principal Investigator
 (434) 220-0159
 danilichm@lunainnovations.com
Business Contact
 Maggie Hudson
Title: Senior Contracts Administ
Phone: (434) 483-4254
Email: submissions305@lunainc.com
Research Institution
 Stub
Abstract
One challenge encountered aboard marine vessels is the treatment, storage, and discharge of wastewater from marine sanitation devices (MSDs). Improperly treated waste water has a high potential for transmitting diseases and harming the ecosystem. The presence of Escherichia coli is routinely used as a key indicator of poor wastewater treatment. Conventional E. coli assays, which are required to detect fewer than 100 bacteria/ml, are laborious selective culture plating methods and require 24-48 hours to complete, increasing onboard storage and sanitation requirements. During Phase I, Luna"s rapid fluorescent viability assays, previously developed for monitoring biothreat bacteria, will be adapted for use with E coli and non-fecal coliforms. To rapidly predict the efficacy of MSDs, we will develop and validate high-throughput syringe formats for detection of coliforms by enzyme hydrolysis of fluorescent substrates. Luna Innovations proposes to i) down select fluorescent assays, ii) optimize enzyme induction, iii) develop a rapid high-through syringe filter format and iv) demonstrate the fluorescent format using fresh MSD wastewater effluents. During Phase II, Luna will i) validate the methods, ii) perform full scale evaluations on Naval vessels, iii) explore potential sources for Phase III support and iv) begin transition to NAVSEA for acquisition and commercialization.

* Information listed above is at the time of submission. *

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