An automated and programmable microfluidic platform for combinatorial gene assembly and biosynthesis applications

Award Information
Agency:
Department of Defense
Amount:
$99,912.00
Program:
STTR
Contract:
D13PC00039
Solitcitation Year:
2012
Solicitation Number:
2012.B
Branch:
Defense Advanced Research Projects Agency
Award Year:
2013
Phase:
Phase I
Agency Tracking Number:
D12B-003-0009
Solicitation Topic Code:
ST12B-003
Small Business Information
HJ Science & Technology, Inc.
187 Saratoga Avenue, Santa Clara, CA, -
Hubzone Owned:
N
Woman Owned:
N
Socially and Economically Disadvantaged:
N
Duns:
968226634
Principal Investigator
 Michael Pollack
 Chief Technology Officer
 (925) 766-3997
 e.jensen@hjsciencetech.com
Business Contact
 Hong Jiao
Title: President
Phone: (408) 464-3873
Email: h.jiao@hjsciencetech.com
Research Institution
 Lawrence Berkeley Nat'l Laboratory
 Susan M Hedley
 One Cyclotron Road
971-SP
Berkeley, CA, 94720-
 (510) 486-6273
 Nonprofit college or university
Abstract
HJ Science & Technology (HJS & T) and the Joint BioEnergy Institute (JBEI) propose to develop an automated, software-controlled, programmable, low-cost, and compact platform capable of running rapid and complex bioengineering processes and optimization of new biomanufacturing systems. Our approach combines the microfluidic automation technology of HJS & T with the novel synthetic biology technologies of combinatorial gene library generation, host transfection, and gene product screening at JBEI. Compared with conventional approaches, the integrated microfluidic technology has these advantages: 1) on-chip automation, eliminating the need for bulky and expensive sample handling robots, 2) small volume, reducing reagent consumption, and 3) multifunctional integration on a microchip level. In Phase I, we will establish the feasibility of the microfluidic automation technology by performing 1) construction of a combinatorial library of Green Fluorescent Protein and Red Fluorescent Protein gene expression cassettes, and 2) subsequent transformation into yeast cells/chromosomal integration and screening of expression products, on the same microfluidic chip in a fully automated format. In Phase II, we will realize a fully automated and programmable platform for the biological design-build-test cycle that encompasses 4 basic steps: 1) software DNA design; 2) DNA synthesis and assembly; 3) transfection/chromosomal integration and product screening; and 4) real-time feedback and control.

* information listed above is at the time of submission.

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