Slip-flow chromatography

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$100,000.00
Award Year:
2013
Program:
STTR
Phase:
Phase I
Contract:
1R41GM106580-01
Award Id:
n/a
Agency Tracking Number:
R41GM106580
Solicitation Year:
2013
Solicitation Topic Code:
NIGMS
Solicitation Number:
PA12-089
Small Business Information
9040 S RITA RD, STE 1100, TUCSON, AZ, 85747-9115
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
Y
Duns:
808276237
Principal Investigator:
MARYWIRTH
(765) 494-5328
mwirth@biovidria.com
Business Contact:
MARYWIRTH
(765) 414-4666
mwirth@biovidria.com
Research Institute:
PURDUE UNIVERSITY

PURDUE UNIVERSITY
1076 FREESHAFER HALL WEST LAFAYETTE, IN 47907-1076
WEST LAFAYETTE, IN, 47907-
() -

Abstract
DESCRIPTION: The product will be a packed capillary for nanoUHPLC of peptides that provides a dramatic increase in speed and efficiency. Current commercial columns for high speed and efficiency use sub-2 mm particles. The proposed technology uses sub-0.2mm particles. The ability to use such small particles for UHPLC is enabled by the phenomenon of slip flow, which gives enhanced volume flow rates in reversed-phase liquid chromatography. Slip flow is a breakthrough that overcomes the fundamental limit to peak broadening caused by the parabolic velocity profile in the mobile phase. Using nonporous sub-0.2 mm particles also eliminates intraparticle diffusion while giving high phase ratios. Preliminary results show that sub micrometer nonporous silica particles reach the diffusion limit for efficiency in protein separations, and the diffusion limit is expected to be reached for the faster diffusing peptides. This gives plate numbers that are independent of length, allowing columns less than 2 cm in lengthto give 100,000 plates with high speed. The specific aims are to 1) optimize the particle diameter, 2) optimize the separation length, and 3) evaluate the nano LC-MS of a tryptic digest. PUBLIC HEALTH RELEVANCE PUBLIC HEALTH RELEVANCE: Aconcept from Nano fluidics called slip flow is introduced to design chromatography columns having greatly improved speed for faster progress in disease diagnostics and therapy.

* information listed above is at the time of submission.

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