Remote focusing through spatial light modulation for multifocal multiphoton micro

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R41MH102201-01
Agency Tracking Number: R41MH102201
Amount: $98,023.00
Phase: Phase I
Program: STTR
Awards Year: 2013
Solicitation Year: 2013
Solicitation Topic Code: NIMH
Solicitation Number: PA12-089
Small Business Information
BOULDER NONLINEAR SYSTEMS, INC.
450 COURTNEY WAY, UNIT 107, LAFAYETTE, CO, -
DUNS: 602673188
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 JAY STOCKLEY
 (303) 604-0077
 jstockley@bnonlinear.com
Business Contact
 MARK TANNER
Phone: (303) 604-0077
Email: mtanner@bnonlinear.com
Research Institution
 COLORADO SCHOOL OF MINES
 COLORADO SCHOOL OF MINES
1500 ILLINOIS ST
GOLDEN, CO, 80401-1887
 () -
 Nonprofit college or university
Abstract
DESCRIPTION (provided by applicant): Nonlinear optical microscopy techniques (such as two-photon florescence) are being used to acquire volumetric (i.e. three dimensional) images that probe several hundred microns into scattering tissue. These techniques are being combined with fast acquisition schemes to allow imaging of live, moving specimens at high NA. This combination allows for high-resolution, in vivo exploration of biological specimens. However, the added system complexity prevents these microcopy techniques from being readily exploited by the biological and medical communities that would greatly benefit from them. Therefore, we propose the development of a simplified confocal microscope configuration that rapidly acquires volumetric images within ascattering media. It uses cost-effective and user-friendly components, meaning they do not require optical, electrical or mechanical expertise to setup and operate. PUBLIC HEALTH RELEVANCE PUBLIC HEALTH RELEVANCE: The successful demonstration ofremote focusing a multifocal, multiphoton microscope using a spatial light modulator as proposed here will, on further development, provide the medical and biological communities a cost effective means of acquiring volumetric images for fundamental studies in the neurosciences. Neurological studies within living systems pose a particularly challenging scenario, requiring detailed, high-resolution (lt1 m) images from deep (up to 1 mm) within the highly scattering specimen. The proposed single element detection technique allows unprecedented, quantitative exploration of dynamics within volumes of living tissue, which directly addresses the imaging challenges confounding this community.

* information listed above is at the time of submission.

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