Probes targeting multiple myeloma in the context of tumor-stromal interactions

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$449,824.00
Award Year:
2013
Program:
SBIR
Phase:
Phase I
Contract:
1R43GM108110-01
Award Id:
n/a
Agency Tracking Number:
R43GM108110
Solicitation Year:
2013
Solicitation Topic Code:
NIGMS
Solicitation Number:
PA11-335
Small Business Information
12521 Gulf Freeway, HOUSTON, TX, 77034-4509
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
788679244
Principal Investigator:
CURTISLAM
(832) 379-2170
curtis.lam@thioaptamer.com
Business Contact:
MARKSHUMBERA
(832) 295-1483
mark.shumbera@thioaptamer.com
Research Institute:
Stub




Abstract
DESCRIPTION: AM Biotechnologies (AM) is developing an easy-to-use kit that enables a technician in a life science laboratory to rapidly develop renewable X-Aptamer (XA) affinity reagents at a reasonable price. Affinity reagents are widely used in research, diagnostic, and clinical applications. Antibodies are currently the most common affinity reagents; however, every aspect of the life science market has experienced problems with them including aggregation, precipitation, difficult quality control, batch-to-batch reproducibility issues, short shelf life, eed for cold storage, costly production, and permanent denaturation. X-Aptamers address every antibody limitation. The X-Aptamer Selection Kit has three basic components: 1) a microbead-based oligonucleotide library; 2) a set of reagents for selecting XAs to a target of interest; and ) a few simple hardware items used for XA selection. X-Aptamers are comprised of a DNA scaffold that incorporates non-DNA functional groups such as amino acid side chains andsmall molecules that interact with a target more robustly than standard DNA. XAs are developed in a proprietary, single-round, bead-based selection process using equipment found in virtually any life science laboratory. The process enables the selection ofchemically-modified affinity reagents that cannot be enzymatically generated, which clears the way for a wide array of chemical functional groups that have never before been used in aptamer-based affinity agent selections. Several chemical functional groups have been tested to date and have resulted in X-Aptamers with low picomolar binding affinity. In addition, using XA processes, the binding affinity of a small molecule was improved by a million-fold. While extraordinary results have been obtain to date,there is a strong need to evaluate and optimize combinations of functional groups that hold the potential for creating protein affinity reagents with unmatched affinity, specificity, physical stability, and robust manufacturability. In this project, AM will develop and evaluate multiple chemically-modified library designs for selecting XAs to diverse protein targets and will develop robust methods for selecting ligand-modified XAs to ligand-binding protein targets. Successful completion of this Phase I project will set the stage for a Phase II project to optimize the X-Aptamer Selection Kit processes for commercialization in Phase III. PUBLIC HEALTH RELEVANCE PUBLIC HEALTH RELEVANCE: The X-Aptamer Selection Kit will enable the life science market to quickly and easily develop chemically-synthesized, renewable affinity reagents that rival the performance of the best biologically-produced antibodies. X-Aptamers will address almost all limitations associated with widely-used antibodies and have the potential to lead to more effective diagnostics as well as new classes of therapeutic drugs.

* information listed above is at the time of submission.

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