Biomarker Quantitation by NP-MS Tissue Imaging.

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$2,954,841.00
Award Year:
2013
Program:
SBIR
Phase:
Phase II
Contract:
2R44DA036263-02
Agency Tracking Number:
R44DA036263
Solicitation Year:
2013
Solicitation Topic Code:
NIDA
Solicitation Number:
PA12-088
Small Business Information
IONWERKS, INC.
3401 Louisiana St., Suite 355, HOUSTON, TX, -
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
154074553
Principal Investigator:
JOHN SCHULTZ
(713) 522-9880
al@ionwerks.com
Business Contact:
SUE EGAN
(713) 522-9880
segan@ionwerks.com
Research Institution:
Stub




Abstract
DESCRIPTION (provided by applicant): MALDI tissue imaging, when enabled by Nanoparticulate (NP) Matrix implantation, is maturing into a useful preclinical tool for biomarker and drug discovery in our laboratory and at NIDA-IRP. Quantitatively reproducibleimages from serial coronal sections suggest that relative quantitation is now possible in rodent models of disease and injury. Nanoparticulate (NP) matrix implantation allows numerical quantitation of differences in biomarker levels in rodent disease and injury models. No commercial instrument which performs mass spectral molecular imaging of tissues has software capable of analyzing and displaying these quality data sets. 1) Software and hardware developed under our antecedent phase II for analyzing the multidimensional data from our MALDI-Ion Mobility-MS imaging system will be expanded to serve this need and will also have dual use in correlating molecular image quality with the nano-scale characterization of the NP matrices by advance materials analysis techniques such as the subnanometer resolution Zeiss Orion Helium Ion Microscope. 2) The continued growth of unique core shell NP matrices will continue to be aided by advanced materials analysis measurement of the NP structures and particle size distributions after implantation into the tissue. 3) Protocols for standards incorporation into and onto the tissue surface will attempt to prove the capability of relative and absolute quantitation in MALDI-MS imaging. PUBLIC HEALTH RELEVANCE PUBLIC HEALTH RELEVANCE: Nanoparticulate (NP) matrix implantation numerical quantitation of differences in biomarker levels in rodent disease and injury models may be possible. No commercial instrument which performs imaging has software capable of analyzing and displaying these data sets. Software developed under a previous phase II for our Ion mobility system can be modified to fulfill these needs for other instruments. Growth of new NPs, advanced characterization, and biological imaging will occur in parallel.

* information listed above is at the time of submission.

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