Development of Antibodies for the Detection of 2-Methylisoborneol (MIB) by Immunoassay

Award Information
Agency: Department of Agriculture
Branch: N/A
Contract: 2002-33610-11945
Agency Tracking Number: 2002-00360
Amount: $79,919.00
Phase: Phase I
Program: SBIR
Awards Year: 2002
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
2935 Byberry Road, Hatboro, PA, 19040
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Fernando Rubio
 () -
 frubio@abraxiskits.com
Business Contact
 Fernando Rubio
Title: President
Phone: () -
Email: frubio@abraxiskits.com
Research Institution
N/A
Abstract
The aquaculture industry considers off-flavor in catfish to be one of the biggest problems facing the industry and representing a significant cost to catfish farmers and processors due to the unmarketability of the fish that have acquired these distasteful compounds. To avoid marketing fish with off-flavor, fish are screened for flavor quality before harvesting. Current quantitative chemical analysis is inadequate for flavor quality control because of time constraints and the high cost involved. Another technique utilizing human flavor testers is sensory analysis, although rapid and relative inexpensive, human assessment is prone to errors due to sickness, fatigue and variability among testers. In this work we propose to develop and produce monoclonal and polyclonal antibodies which react with 2-methylisoborneol (MIB) with sufficient selectivity, affinity, and avidity to be utilized in a low cost and rapid commercial immunoassay system, which ultimately benefit farmers, processors and consumers by ensuring a high quality product. Production of these antibodies is described including the immunogens, injection schedule, screening process, hybridoma development, polyclonal and monoclonal selection criteria.

* Information listed above is at the time of submission. *

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