SBIR Phase I: New Fluorescent Biosensors for Signaling in Living Cells

Award Information
Agency: National Science Foundation
Branch: N/A
Contract: 1248138
Agency Tracking Number: 1248138
Amount: $149,423.00
Phase: Phase I
Program: SBIR
Awards Year: 2013
Solicitation Year: 2012
Solicitation Topic Code: BC
Solicitation Number: N/A
Small Business Information
Montana Molecular LLC
619 N. Church Ave, Unit 4, Bozeman, MT, 59715-3087
DUNS: 145280157
HUBZone Owned: Y
Woman Owned: Y
Socially and Economically Disadvantaged: N
Principal Investigator
 Anne Quinn
 (406) 539-7399
 amq@montanamolecular.com
Business Contact
 Anne Quinn
Phone: (406) 539-7399
Email: amq@montanamolecular.com
Research Institution
 Stub
Abstract
This Small Business Innovation Research (SBIR) Phase I project will establish the feasibility of developing a genetically-encoded biosensor to monitor levels of cyclic adenosine monophosphate (cAMP), an important second messenger component of drug signaling pathways. Unlike existing FRET-based biosensors that depend upon energy transfer between two fluorescent molecules, this sensor will employ a single, circularly permuted fluorescent green protein. This green sensor can be coupled with differently colored biosensors for other second messengers to produce simultaneous readouts for multiple components of a signaling pathway that change when activated by a drug. The broader impact/commercial potential of this project is that multiplex, genetically-encoded assays reporting multiple cell signaling events would expand the depth of knowledge about signal transduction by improving information on the timing, location and pathway cross-talk in physiologically relevant tissues. These assays are homogenous, do not require multiple steps, or cell lysis. A growing trend in the pharmaceutical industry is screening in primary cell cultures, and genetically encoded assays are ideally suited for this. The technology to be developed in this proposal represents a new innovation in fluorescent live-cell assay and has strong potential to reduce the cost and improve the reliability of drug discovery to find safe and effective drugs that provide better treatment outcomes and improved human health.

* information listed above is at the time of submission.

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