An automated, high throughput, resin-free device for large scale protein purification

Award Information
Agency:
Department of Defense
Branch
Office for Chemical and Biological Defense
Amount:
$494,684.00
Award Year:
2014
Program:
SBIR
Phase:
Phase II
Contract:
W911NF-14-C-0068
Award Id:
n/a
Agency Tracking Number:
C2-0383
Solicitation Year:
2012
Solicitation Topic Code:
CBD12-102
Solicitation Number:
2012.2
Small Business Information
701 McMillian Way, NW, Suite D, Huntsville, AL, 35806-
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
Y
Duns:
185169620
Principal Investigator:
HongjunSong
Research Engineer
(256) 726-4904
proposals-contracts@cfdrc.com
Business Contact:
DeborahPhipps
Contracts Manager
(256) 726-4884
dap@cfdrc.com
Research Institute:
n/a
Abstract
Protein manufacturing is of paramount importance to chemical and biological defense applications. Existing protein purification methods primarily rely on synthetic-resin based chromatography, which is time-consuming, labor-intensive, expensive, and consequently, ill-suited for developing rapid countermeasures to chemical and biological threats. To overcome these limitations, the overall goal of this effort is to develop a novel high-throughput, resin-free technology for automated protein recovery and purification. Our technology is based on multi-scale electrokinetics and enables order-of-magnitude improvement in investment and operating cost. In Phase I, we designed, fabricated, characterized, and demonstrated a pilot-scale prototype to establish proof-of-principle of the proposed technology. The designs of the purification device were first evaluated using high-fidelity, multi-physics simulations, followed by prototype fabrication and engineering. The assembled prototypes were extensively tested and salient protein purification performance similar to the traditional resin based methods was demonstrated, but with the benefits of resin-free operation, reduced analysis time, ease of operation, and significantly lower cost. In Phase II, design optimization and protocol refinement will be carried out for enhanced performance, manufacturability, and cost effectiveness. The most promising purification mechanisms will be selected for device scale up and integration with COTS technologies. The integrated prototype will be demonstrated for purification of DoD-relevant proteins in composite samples from various expression systems. The Phase II end-product will be a fully automated, high throughput and low cost system for rapid protein purification, which will be commercialized to meet the needs in both military and civilian markets.

* information listed above is at the time of submission.

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