MULTIWELL COLORIMETRIC ASSAY FOR DNA REPAIR PROTEIN.

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R43CA096392-01A1
Agency Tracking Number: CA096392
Amount: $99,380.00
Phase: Phase I
Program: SBIR
Awards Year: 2002
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
ACTIVE MOTIF, LLC
ACTIVE MOTIF, LLC, 5431-C AVENIDA ENCINAS, CARLSBAD, CA, 92008
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 JACQUES WEISSMAN
 () -
Business Contact
 KENT JOHNSON
Phone: (760) 431-1263
Email: JOHNSON@ACTIVEMOTIF.COM
Research Institution
N/A
Abstract
DESCRIPTION (provided by applicant): DNA damages are key events during carcinogenesis. Deficiencies in repairing damaged DNA represent a poor prognosis during tumorigenesis and/or cancer treatment. Besides monitoring DNA damage formation, it would be helpful to monitor DNA repair protein activity. We propose to develop a new technology that allows for high-throughput and fast analysis of the most common DNA repair proteins. We will use a damaged DNA probe immobilized onto a 96-well microplate to capture DNA repair proteins in an extract from cells or tissues. Six biotinylated oligonucleotides containing different DNA damage types will be synthesized. The DNA-protein binding conditions will be developed by using purified recombinant DNA repair proteins. Antibodies raised specifically against DNA repair proteins will identify the bound protein and yield a quantitative result in two hours. Finally, the procedure will be applied to relevant samples such as extracts from damaging agent-treated cell lines and disease models. Such assay will help elucidate the molecular mechanisms of the DNA damage and repair pathways. It would also provide scientists and clinicians with a unique tool to answer seminal questions in risk assessment in a fraction of the time normally required.

* information listed above is at the time of submission.

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