FLUOROGENIC IMAGING MOLECULES FOR PROTEASE/KINASE ASSAYS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$99,145.00
Award Year:
2002
Program:
SBIR
Phase:
Phase I
Contract:
1R43CA096423-01
Award Id:
60027
Agency Tracking Number:
CA096423
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
ACTIVE MOTIF, LLC, 5431-C AVENIDA ENCINAS, CARLSBAD, CA, 92008
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
JOHNARCHDEACON
(760) 431-1263
ARCHDEACON@ACTIVEMOTIF.COM
Business Contact:
KENNETHJOHNSON
(760) 431-1263
JOHNSON@ACTIVEMOTIF.COM
Research Institute:
n/a
Abstract
DESCRIPTION (provided by applicant): The aim of this proposal is to develop a series of fluorogenic imaging molecules, which can be easily delivered into viable cells and monitor cellular functions. The FIMs are composed of a peptide backbone containing a target site for a kinase or a protease. Some designs borrow from the molecular beacon approach and carry flurophore/quencher moieties, while other designs simply carry a flurophore only. When present, the quencher moiety is internal to the FIM rather than at the extreme N- and C-terminus, as has been the case with other similar type beacons. This avoids the need for structural constraint, which aims to be imposed in order to bring the flurophore and quencher into close proximity to each other thereby preventing background fluorescence. Specifically, the scope of this proposal will cover the design, synthesis and testing of two peptides containing cleavage sites specific for caspase-1 (ICE) and caspase-3 (CPP32). Also included in this proposal is the design, synthesis and testing of fluorogenic peptides derived from a differentially phosphorylated, essential domain of human cdd25C phosphatase, which is involved in induction of the mitotic process.

* information listed above is at the time of submission.

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