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Innovative Nucleic Acids to Diagnose Endemic Fungal Infections

Award Information
Agency: Department of Health and Human Services
Branch: Centers for Disease Control and Prevention
Contract: 200-2014-M-60866
Agency Tracking Number: 200-2014-M-60866
Amount: $150,000.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 2014
Award Start Date (Proposal Award Date): 2014-09-01
Award End Date (Contract End Date): 2015-03-15
Small Business Information
13709 Progress Blvd N 104
Alachula, FL 32615-
United States
DUNS: 192849011
HUBZone Owned: Unavailable
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Dennis  Kristof
 (386) 418-0347
 dkristof@firebirdbio.com
Business Contact
 Dennis  Kristof
Phone: (386) 418-0347
Email: dkristof@firebirdbio.com
Research Institution
N/A
Abstract

Firebird will deliver multiplexed assays that inexpensively detect and distinguish coccidioidomycosis, blastomycosis, and histoplasmosis by targeting the genomic DNA of their causative agents. This assay uses six Firebird technologies:
1. Artificially expanded genetic information systems (AEGIS), which support ultra-clean DNA amplification with ultra-low noise despite the complexity of realbiological samples.
2. Self-avoiding molecular recognition systems (SAMRS), which permit unlimited multiplexing of xNA­
targeted assays, and (consequently) low cost and extreme adaptability in such assays.
3. Conversion technologies that create AEGIS-containing amplicons easily detected by Luminex without interference from any naturally found xNA.
4. Evolution-based software to select genomic targets for genus and species discrimination.
5. Novel reagent-enzyme combinations that make fungal xNA accessible to amplification.
6. Novel isothermal amplification methods to allow the assays to work near points-of-care.
The initial product will address the interest of the NCEZID in analyzing environmental samples, to avoid costly FDA regulation. Firebird's investment in pipelines allows us to prepare AEGIS and SAMRS building blocks in multi-gram amounts, and their oligonucleotides on demand. Thus, this product fits well within Firebird's current product line. Specific diagnosis of fungal infections will enhance public health by providing a mechanism to save lives and prevent death serious disease.

* Information listed above is at the time of submission. *

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