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Spiral Countercurrent Chromatography for High-throughput Natural Product Purifica

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R43AT008296-01
Agency Tracking Number: R43AT008296
Amount: $217,391.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: NCCIH
Solicitation Number: AT14-001
Timeline
Solicitation Year: 2014
Award Year: 2014
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
12111 PARKLAWN DRIVE #125
ROCKVILLE, MD 20852-1707
United States
DUNS: 788824337
HUBZone Owned: No
Woman Owned: Yes
Socially and Economically Disadvantaged: No
Principal Investigator
 MARTHA KNIGHT
 (240) 669-9281
 marthaknight@starpower.net
Business Contact
 MARTHA KNIGHT
Phone: (240) 207-2060
Email: marthaknight@starpower.net
Research Institution
 Stub
Abstract

DESCRIPTION (provided by applicant): Recent advances in countercurrent chromatography (CCC) have created novel spiral-design separation columns that hold a very high stationary phase of all the solvent systems, such that now all molecules of any size and water solubility can be successfully separated in high-speed countercurrent chromatography. With spiral CCC there is inherent versatility, more than any type of chromatography. Preliminary studies with the new spiral tubing support rotor succeeded in purifying Sutherlandioside B, a major water soluble cycloartane glycoside from Lessertia frutescent (Sutherlandia) is using an n- butanol solvent system. With the small volume of the coil (~100 ml) and a stationary phase at 50% (~50 ml), it is impressive that amass of 1.2 g of crude plant extract could be efficiently fractionated in one run with high recovery of the target compound. In addition, the active iridoid glycoside, harpagoside was isolated from an extract of Harpagophytum procumbens (Devil's Claw

* Information listed above is at the time of submission. *

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