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Direct RT-qPCR analysis of microRNAs in human plasma (miR-Direct)

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R43CA177074-01A1
Agency Tracking Number: R43CA177074
Amount: $225,000.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: NCI
Solicitation Number: PA13-088
Timeline
Solicitation Year: 2014
Award Year: 2014
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
2161 DELAWARE AVE
SANTA CRUZ, CA 95060-5790
United States
DUNS: 13494781
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 SERGEI KAZAKOV
 (831) 426-7700
 skazakov@somagenics.com
Business Contact
 BRIAN JOHNSTON
Phone: (831) 426-7700
Email: bjohnston@somagenics.com
Research Institution
 Stub
Abstract

Our goal in this proposal is to develop a novel method for the direct detection of miRNAs in serum and plasma without requiring isolation of total RNA. Distinct expression profiles of microRNAs (miRNAs) have recently been associated with cancer and other diseases, implying that miRNAs could serve as diagnostic biomarkers for these diseases. Effective biomarkers could facilitate early detection of cancer, leading to improved therapeutic outcomes, and aid in monitoring progression and response to therapy. TheRT-qPCR assays currently employed to quantify circulating miRNAs are hampered by problems associated with their requirement for isolating total RNA from plasma or serum prior to miRNA quantification. RNA is typically isolated by spin-column purification or phenol extraction and ethanol precipitation. Both methods suffer from inconsistent miRNA recovery and the lack of an internal reference miRNA for data normalization. Moreover, current RNA isolation procedures do not completely remove inhibitors of enzy

* Information listed above is at the time of submission. *

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