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Minimal piggyBac vectors for chromatin integration

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R41GM109527-01
Agency Tracking Number: R41GM109527
Amount: $150,576.00
Phase: Phase I
Program: STTR
Solicitation Topic Code: NIGMS
Solicitation Number: PA13-089
Timeline
Solicitation Year: 2014
Award Year: 2014
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
4526 KINGSWOOD DR
MOBILE, AL 36608-2814
United States
DUNS: 968503164
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 VICTOR SOLODUSHKO
 (251) 460-7489
 vsolodushko@southalabama.edu
Business Contact
 CHRISTINE CUMBIE
Phone: (251) 401-5186
Email: ccumbie@gardberglaw.com
Research Institution
 UNIVERSITY OF SOUTH ALABAMA
 
UNIVERSITY OF SOUTH ALABAMA 307 N University Blvd, AD200
MOBILE, AL 36688-0002
United States

 () -
 Nonprofit college or university
Abstract

In this grant, we describe novel transposon piggyBac vectors engineered to deliver transgenes as efficiently as currently available piggyBac systems, but with significantly less helper DNA co-delivered into the host genome. To generate these plasmids, we first identified an important and previously unreported aspect of transposon biology, that the full-length terminal domains required for successful plasmid-to-chromatin transgene delivery can be removed from the transgene delivery cassette without impairingtransposition efficiency, as long as these terminal domains are present somewhere within the plasmid. Transposons have the capacity to carry a large cargo load and therefore represent an alternative to retroviral and lentiviral delivery of genes. While transposons do not generate an immune response like retroviruses, current transposons still deposit large amounts of helper DNA into the host genome, DNA that can produce long-term problems due to its retained enhancer and promoter activity. In this grant,

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