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Amplification-free megabase target-enrichment for next-generation sequencing

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R41HG008117-01
Agency Tracking Number: R41HG008117
Amount: $215,000.00
Phase: Phase I
Program: STTR
Solicitation Topic Code: NHGRI
Solicitation Number: PA13-235
Timeline
Solicitation Year: 2014
Award Year: 2014
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
953 Indiana St.
San Francisco, CA 94107-3007
United States
DUNS: 968684261
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 DENNIS EASTBURN
 (415) 513-7728
 dennis.eastburn@gmail.com
Business Contact
 DENNIS EASTBURN
Phone: (415) 513-7728
Email: dennis.eastburn@gmail.com
Research Institution
 UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
 
UNIVERSITY OF CALIFORNIA SAN FRANCISCO 3333 California Street., Ste 315
SAN FRANCISCO, CA 94143-0962
United States

 () -
 Nonprofit college or university
Abstract

DESCRIPTION (provided by applicant): Target enrichment for next-generation sequencing enables researchers to reduce the burden of sequence data analysis, obtain higher levels of statistical significance by examining more genomes and increase sequencing coverage depth. The goal of this STTR proposal is to develop and commercialize a novel technology capable of amplification-free megabase target sequence enrichment. This technology is highly differentiated from all other enrichment approaches currently on themarket. Our method, MESA (Megabase Enrichment Sans Amplification), isolates large megabase-sized genomic DNA molecules in microfluidic drops and performs TaqMan PCR reactions to confirm the presence of the desired target sequence. The TaqMan identified drops are then sorted and collected to enrich for the target sequence prior to DNA sequencing. This approach is unique in that it is the only method that isolates intact megabase-sized fragments and does so without amplification of the DNA. The TaqMan P

* Information listed above is at the time of submission. *

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