Detection of Hybridization Without Radioactive Label

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1993
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
22097
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Adelphi Technology Inc.
2181 Park Boulevard, Palo Alto, CA, 94306
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Qiang Li
(415) 328-7337
Business Contact:
() -
Research Institution:
n/a
Abstract
A novel surface phenomenon will be explored for the quantitative detection of nucleic acid hybridization. We will construct a relatively inexpensive instrument, which can attain a sensitivity limit comparable to the existing methodologies, without the use of dyes or radioisotopes. As part of the Phase I research, we will use the existing hardware to test affinity of DNA to a specifically prepared surface. This test is a necessary step in the operation of the instrument. We will also measure the binding of the complementary DNA to assess the detection limit, sensitivity and the issue of non-specific binding. In Phase II, the signal to noise ratio will be optimized, and a prototype of the device specifically designed for hybridization detection will be built. The complete hybridization protocol will be tested on the new instrument. If the research is successful, the new instrument can perform routine nucleic acid hybridization detection for molecular biology research and clinical laboratory tests.

* information listed above is at the time of submission.

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