Ethanol Production via Consolidated Bioprocessing

Award Information
Agency: Department of Energy
Branch: N/A
Contract: DE-FG02-03ER83593
Agency Tracking Number: 72808S03-I
Amount: $749,827.00
Phase: Phase II
Program: SBIR
Awards Year: 2004
Solicitation Year: 2003
Solicitation Topic Code: 22
Solicitation Number: DOE/SC-0059
Small Business Information
99 1/2 School Street, Lebanon, NH, 03766
HUBZone Owned: Y
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Michael Tyurin
 (603) 646-9212
Business Contact
 Jonathan Mielenz
Title: Dr.
Phone: (603) 646-9321
Research Institution
72808-The conversion of cellulosic biomass to commodity products via biological processing could benefit society by improving energy security, reducing greenhouse gas emissions, and promoting rural economies. However, commercial application is impeded by the current absence of low-cost technology to overcome the recalcitrance of cellulosic biomass. This project will develop a strain of Clostridium thermocellum, a rapid cellulose-fermenting microorganism that produces ethanol as well as organic acids, for producing industrial ethanol from pretreated biomass in a consolidated bioprocessing (CBP) configuration. Phase I established the technical capability to metabolically engineer C. thermocellum. A high efficiency gene transfer system for this organism, using custom apparatus and an optimized protocol, was developed. Site-specific gene integration into genes associated with acetic acid formation was demonstrated. Fermentation studies showed a dramatic decrease in acetic acid production compared to the wild-type C thermocellum. Phase II will develop engineered strains of C. thermocellum that produce ethanol at high yield, along with commercially recoverable concentrations from pretreated corn stover in the absence of added saccharolytic enzymes. Particular objectives include: (1) demonstrating the efficacy and removal of additional genetic markers; (2) developing C. thermocellum strains that produce ethanol at high yield; (3) developing ethanol-tolerant C. thermocellum strains; (4) demonstrating the effectiveness of AFEX pretreatment for C. thermocellum fermentation; and (5) analyzing process economics and business opportunities. Commercial Applications and Other Benefits as described by awardee: The technology should expand fuel production from indigenous, renewable resources, and also establish a process concept that can be applied to the production of industrial chemicals.

* Information listed above is at the time of submission. *

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