Ethanol Production via Consolidated Bioprocessing

Award Information
Agency:
Department of Energy
Branch
n/a
Amount:
$749,827.00
Award Year:
2004
Program:
SBIR
Phase:
Phase II
Contract:
DE-FG02-03ER83593
Award Id:
61715
Agency Tracking Number:
72808S03-I
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
99 1/2 School Street, Lebanon, NH, 03766
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Michael Tyurin
Dr.
(603) 646-9212
mtyurin@hotmail.com
Business Contact:
Jonathan Mielenz
Dr.
(603) 646-9321
jmielenz343@earthlink.net
Research Institution:
n/a
Abstract
72808-The conversion of cellulosic biomass to commodity products via biological processing could benefit society by improving energy security, reducing greenhouse gas emissions, and promoting rural economies. However, commercial application is impeded by the current absence of low-cost technology to overcome the recalcitrance of cellulosic biomass. This project will develop a strain of Clostridium thermocellum, a rapid cellulose-fermenting microorganism that produces ethanol as well as organic acids, for producing industrial ethanol from pretreated biomass in a consolidated bioprocessing (CBP) configuration. Phase I established the technical capability to metabolically engineer C. thermocellum. A high efficiency gene transfer system for this organism, using custom apparatus and an optimized protocol, was developed. Site-specific gene integration into genes associated with acetic acid formation was demonstrated. Fermentation studies showed a dramatic decrease in acetic acid production compared to the wild-type C thermocellum. Phase II will develop engineered strains of C. thermocellum that produce ethanol at high yield, along with commercially recoverable concentrations from pretreated corn stover in the absence of added saccharolytic enzymes. Particular objectives include: (1) demonstrating the efficacy and removal of additional genetic markers; (2) developing C. thermocellum strains that produce ethanol at high yield; (3) developing ethanol-tolerant C. thermocellum strains; (4) demonstrating the effectiveness of AFEX pretreatment for C. thermocellum fermentation; and (5) analyzing process economics and business opportunities. Commercial Applications and Other Benefits as described by awardee: The technology should expand fuel production from indigenous, renewable resources, and also establish a process concept that can be applied to the production of industrial chemicals.

* information listed above is at the time of submission.

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