Genetic Assay for Inhibitors of RNA-Protein Interactions

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 2R42CA103407-02
Agency Tracking Number: CA103407
Amount: $820,375.00
Phase: Phase II
Program: STTR
Awards Year: 2005
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Advanced Genetic Systems, Inc.
Advanced Genetic Systems, Inc., 1554 25Th Ave, San Francisco, CA, 94122
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 ALAN FRANKEL
 (415) 476-9994
 FRANKEL@CGL.UCSF.EDU
Business Contact
 ROBERT NAKAMURA
Phone: (415) 806-0187
Email: ROBNAK@ADVANCEDGENETICSYSTEMS.COM
Research Institution
 UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
 UNIVERSITY OF CALIFORNIA SAN FRANCISCO
3333 California St., Ste. 315
SAN FRANCISCO, CA, 94143
 Nonprofit college or university
Abstract
RNA-protein interactions play critical roles in cellular regulatory processes and in the life cycles of many pathogenic viruses including HIV, hepatitis C, SARS, and West Nile virus, and thus represent attractive targets for therapeutics. We have developed novel cell-based assays to identify drugs that target the RNA-protein interactions. The assays incorporate a system of genetic reporters to accurately recapitulate RNA-protein interactions and enable screens with high sensitivity and specificity. In an initial test of the system, the HIV Rev-RRE interaction was successfully targeted with control inhibitors of the RNA-protein interaction. Experiments to optimize and convert the drug screening assays to high throughput format are currently in progress. The assays will be tested by screening a combinatorial library of RRE-binding peptides to be followed by a high-throughput chemical compound library screen. The development of high-throughput genetic methods to identify specific inhibitors of RNA-protein interactions is expected to help in the discovery of drugs directed against novel therapeutic targets.

* information listed above is at the time of submission.

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