Genetic Assay for Inhibitors of RNA-Protein Interactions

Award Information
Agency:
Department of Health and Human Services
Branch:
N/A
Amount:
$100,000.00
Award Year:
2003
Program:
STTR
Phase:
Phase I
Contract:
1R41CA103407-01
Agency Tracking Number:
CA103407
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Advanced Genetic Systems
136 S WOLFE RD, 136 S WOLFE RD, Sunnyvale, CA, 94086
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
N/A
Principal Investigator
 ALAN FRANKEL
 (415) 476-9994
 FRANKEL@CGL.UCSF.EDU
Business Contact
 ROBERT NAKAMURA
Phone: (415) 502-8442
Email: ROBNAK@ADVANCEDGENETICSYSTEMS.COM
Research Institution
 UNIVERSITY OF CALIFORNIA SAN FRANCISCO
 500 PARNASSUS AVE
SAN FRANCISCO, CA, 94122
 Nonprofit college or university
Abstract
DESCRIPTION (provided by applicant): RNA-protein interactions play critical roles in cellular regulatory processes and in the life cycles of many pathogenic viruses, including HIV, hepatitis C, and West Nile virus, and thus represent attractive targets for therapeutics. Much of our previous work has centered on identifying and characterizing the components of key interactions and developing cell-based reporters to accurately monitor RNA-protein interactions. Here we focus on developing targeted assays for inhibitor screening. The cell-based screens described incorporate a system of genetic reporters designed to recapitulate RNA-protein interactions in a native mammalian environment and to enable screens with high sensitivity and specificity. As proof-of-principle, reporters cell lines will be developed and used to identify inhibitors of the HIV Rev-Rev Response Element (RRE) interaction from combinatorial zinc finger libraries. The development of high-throughput genetic methods to identify specific inhibitors of RNA-protein interactions is expected to help in the discovery of drugs directed against novel therapeutic targets.

* information listed above is at the time of submission.

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