Genetic Assay for Inhibitors of RNA-Protein Interactions

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$100,000.00
Award Year:
2003
Program:
STTR
Phase:
Phase I
Contract:
1R41CA103407-01
Award Id:
65247
Agency Tracking Number:
CA103407
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
136 S WOLFE RD, 136 S WOLFE RD, Sunnyvale, CA, 94086
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
ALAN FRANKEL
(415) 476-9994
FRANKEL@CGL.UCSF.EDU
Business Contact:
ROBERT NAKAMURA
(415) 502-8442
ROBNAK@ADVANCEDGENETICSYSTEMS.COM
Research Institute:
UNIVERSITY OF CALIFORNIA SAN FRANCISCO

500 PARNASSUS AVE
SAN FRANCISCO, CA, 94122

Nonprofit college or university
Abstract
DESCRIPTION (provided by applicant): RNA-protein interactions play critical roles in cellular regulatory processes and in the life cycles of many pathogenic viruses, including HIV, hepatitis C, and West Nile virus, and thus represent attractive targets for therapeutics. Much of our previous work has centered on identifying and characterizing the components of key interactions and developing cell-based reporters to accurately monitor RNA-protein interactions. Here we focus on developing targeted assays for inhibitor screening. The cell-based screens described incorporate a system of genetic reporters designed to recapitulate RNA-protein interactions in a native mammalian environment and to enable screens with high sensitivity and specificity. As proof-of-principle, reporters cell lines will be developed and used to identify inhibitors of the HIV Rev-Rev Response Element (RRE) interaction from combinatorial zinc finger libraries. The development of high-throughput genetic methods to identify specific inhibitors of RNA-protein interactions is expected to help in the discovery of drugs directed against novel therapeutic targets.

* information listed above is at the time of submission.

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