Novel one-step purification with a sortase fusion

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R43GM074422-01
Agency Tracking Number: GM074422
Amount: $100,000.00
Phase: Phase I
Program: SBIR
Awards Year: 2005
Solicitation Year: 2005
Solicitation Topic Code: N/A
Solicitation Number: PHS2005-2
Small Business Information
ADVANCED PROTEIN LABS
Advanced Protein Labs, 505 Coast Blvd S, Ste 412, La Jolla, CA, 92037
DUNS: N/A
HUBZone Owned: N
Woman Owned: Y
Socially and Economically Disadvantaged: Y
Principal Investigator
 HONGYUAN MAO
 (858) 754-3014
 HMAO@SAN.RR.COM
Business Contact
 HONGYUAN MAO
Phone: (858) 754-3014
Email: HMAO@SAN.RR.COM
Research Institution
N/A
Abstract
DESCRIPTION (provided by applicant): A new protein fusion expression system was conceptualized and is being developed to provide tag-free recombinant protein purification via one-step chromatography. Phase I of this project includes optimization of the activity of the key component in the fusion construct, streamlining the target cloning method, and applications in bacteria expression system. Because this purification method is simple, low cost, time saving, Phase II of this project will advance the one-step method for eukaryotic expression and apply the method to producing proteins for proteomic studies and biotransformation applications.

* information listed above is at the time of submission.

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