Kits for 3-Dimensional Histophysiologic Gradient Culture

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$98,417.00
Award Year:
1995
Program:
SBIR
Phase:
Phase I
Contract:
1 R43 CA68873-01,
Award Id:
29255
Agency Tracking Number:
29255
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
1933 Davis Street, Suite 310, San Leandro, CA, 94577
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Joseph Leighton
() -
Business Contact:
() -
Research Institution:
n/a
Abstract
We will develop a commercial kit so that biologists can readily study tissues under conditions of hgradient culture. Here, as in nature, stratified epithelia adhere to their supporting stroma at a reRenewal of epithelium occurs adjacent to the interface, and all metabolic exchange is by diffusion aculture, a thin, transparent, permeable collagen membrane serves as a "stroma." Cells attach to onethe medium bathes the opposite side, and metabolic exchange for the cells is by diffusion across thethe membrane in contract with the cells is waffled with collagen, which increases the tensile strengthe surface area for cell attachment. The membrane is used in two configurations, as a flat substratchamber closed at both ends. With commercial kits available, a number of basic and clinical biomedicfollow. Processes of tissue remodeling, aging, metaplasia,atrophy, hypertrophy, dysplasia, carcinogeinvasion, and viral propagation can be reconstituted and manipulated. Clinical applications are numediagnosis and treatment,e.g. characterization of cells from a biopsy that will predict the subsequen

* information listed above is at the time of submission.

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