Phage Array Biosensor for Detection of Biowarfare Agents

Award Information
Agency:
Department of Defense
Branch
Army
Amount:
$100,000.00
Award Year:
2001
Program:
STTR
Phase:
Phase I
Contract:
DAAD19-02-C-0017
Award Id:
52987
Agency Tracking Number:
A013-0055
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
P.O. Box 80010, Austin, TX, 78708
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
022552900
Principal Investigator:
Tom Klem
Senior Scientist
(607) 255-8479
tklem@agavebio.com
Business Contact:
Noe Salazar
President
(512) 671-1369
nsalazar@agavebio.com
Research Institute:
CORNELL UNIV.
Linda Brainard
120 Day Hall
Ithaca, NY, 14853
(607) 255-7123
Nonprofit college or university
Abstract
Agave BioSystems, in collaboration with Professor George Malliaras of Cornell University, proposes to develop a unique and innovative biosensor based on induced luminescence of captured BW bacterial agents and organic light emitting diode (OLED)technology. The system would use an array of bacteriophage engineered to express fluorescent protein in infected BW agents. The specificity of the phage provides capture of only targets of interest, while the infection of the bacteria and naturalreplication of the expressed protein will provide the detection signal. Using novel OLED arrays, a phage array chip can be constructed similar to DNA chips for multianalyte detection. The combination of the phage array approach with OLED detectionallows development of a powerful biosensing system that does not require additional labeling, sample manipulation, or sophisticated microfluidic and pumping mechanisms.Potential markets include the food processing, environmental, medical and agriculturalsectors. Relevant examples include the detection of Listeria monocytogenes in dairy foods and detection of multi-drug resistant bacteria in hospitals and clinics. All bacteria responsible for these outbreaks are susceptible to phage infection, and thus arelikely candidates for detection by the phage array biosensor.

* information listed above is at the time of submission.

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