Respiratory Endothelial Cell Sensor for Real-Time Air Toxicity Monitoring

Award Information
Agency:
Department of Defense
Branch
Army
Amount:
$730,000.00
Award Year:
2004
Program:
SBIR
Phase:
Phase II
Contract:
W81XWH-04-C-0043
Award Id:
68271
Agency Tracking Number:
A032-3868
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
P.O. Box 80010, Austin, TX, 78708
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
022552900
Principal Investigator:
TheresaCurtis
Staff Scientist
(607) 272-0002
tcurtis@agavebio.com
Business Contact:
NoeSalazar
President
(512) 671-1369
nsalazar@agavebio.com
Research Institute:
n/a
Abstract
Because many chemical and biological toxins selectively target respiratory endothelial cells, a biosensor using these cells in combination with Electric Cell-substrate Impedance Sensing (ECIS) technology has many useful applications for detection and early warning monitoring of a broad range of toxic agents. In the Phase I SBIR program, Agave BioSystems was highly successful in demonstrating that endothelial cells are a robust system, which can be harnessed in a new class of extremely sensitive yet versatile biosensors to detect a wide variety of chemical toxins. Through simple monitoring of the integrity of respiratory endothelial monolayers using ECIS technology, we successfully detected paraquat, pentachlorophenol, and half-mustard contamination within minutes of addition to the biosensor. Additionally, by combining the respiratory endothelial cells with state of the art microfluidics we were able to continuously deliver samples to the biosensor and demonstrate real-time toxicity monitoring over a two-week period. The focus of the Phase II plan will be to test the system with a wide range of toxic chemicals, refine the microfluidic biochip for continuous flow conditions, develop a field portable ECIS biosensor and integrate an air sampling system for continuous monitoring.

* information listed above is at the time of submission.

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