Combinatorial Molecular Array Analysis via MEF/MALDI/MS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$99,510.00
Award Year:
2004
Program:
SBIR
Phase:
Phase I
Contract:
1R43CA110680-01
Agency Tracking Number:
CA110680
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
AGAVE BIOSYSTEMS
AGAVE BIOSYSTEMS, BOX 80010, AUSTIN, TX, 78708
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
LOUIS HOM
(607) 272-0002
LHOM@AGAVEBIO.COM
Business Contact:
(512) 671-1369
Research Institution:
n/a
Abstract
DESCRIPTION (provided by applicant): Despite numerous advances in high throughput screening (HTS) and system automation, the identification of specific ligands remains time consuming and resource intensive. A major obstacle to more efficient screening of potential ligands is the current requirement for each target protein to be screened individually. A highly effective method for identifying proteins bound to solid supports is matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). But variability in protein-matrix interactions can make accurate assessments of protein binding difficult, since the absence of mass signal could signify either the absence of binding or the poor desorption/ionization behavior of a particular protein/matrix pair. This ambiguity can be resolved, however, by employing a second, independent measure of binding that is not subject to the same protein-to-protein variability. To this end, we propose the development of a novel discovery platform for the combinatorial analysis of molecular arrays, incorporating metal enhanced fluorescence (MEF) to detect protein binding, and MALDI mass spectrometry to identify the proteins involved.

* information listed above is at the time of submission.

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