Detection of Explosive Materials Using an Encapsulated Fluorescent Bioprobe

Award Information
Agency:
Department of Defense
Branch
Army
Amount:
$100,000.00
Award Year:
2006
Program:
STTR
Phase:
Phase I
Contract:
W9132V-06-C-0027
Award Id:
77852
Agency Tracking Number:
A064-025-0256
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
P.O. Box 80010, Austin, TX, 78708
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
022552900
Principal Investigator:
Joel Tabb
(607) 272-0002
jtabb@agavebio.com
Business Contact:
Noe Salazar
President
(512) 671-1369
nsalazar@agavebio.com
Research Institute:
UNIVERSITIES SPACE RESEARCH ASSOC.
Randy Vander Wal
USRA c/o The NCSER
Cleveland, OH, 44135
(216) 433-9065
Nonprofit college or university
Abstract
The use of explosives over the last 100 years has left up to 16,000 military ranges contaminated with highly toxic compounds. A major challenge to the remediation of these ranges is identifying the location of persistent explosive residues. To meet this challenge, Agave BioSystems in collaboration with Universities Space Research Agency (USRA) proposes to develop an encapsulated fluorescent bioprobe for detection of explosive materials. Encapsulation of the bioprobe will rely on porous silica nanoparticles, which will provide a matrix for the probe and enhance its environmental stability by allowing chemical agents of interest to enter while leaving the bioprobe impervious to other compounds and pH fluctuations. Detection will rely on a displacement immunoassay that results in fluorescence in the presence of explosives. Dye-labeled anti-TNT antibody fragments will provide fluorescence, and antibody fragments will be attached within porous silica nanoparticles. In the absence of TNT, the TNT quencher analogue will bind to the dye-labeled antibody fragments, preventing fluorescence. When TNT is present, the TNT quencher analogue will be displaced, removing the fluorescence quenching effect. The Phase I effort will focus on encapsulating the bioprobe and detecting 2,4,6-trinitrotoluene (TNT).

* information listed above is at the time of submission.

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