Dipstick Assay for Field Detection of Leishmania Parasites using Quantum Dots

Award Information
Agency: Department of Defense
Branch: Army
Contract: W81XWH-05-C-0094
Agency Tracking Number: O043-H08-3058
Amount: $100,000.00
Phase: Phase I
Program: SBIR
Awards Year: 2005
Solitcitation Year: 2004
Solitcitation Topic Code: OSD04-H08
Solitcitation Number: 2004.3
Small Business Information
AGAVE BIOSYSTEMS, INC.
P.O. Box 80010, Austin, TX, 78708
Duns: 022552900
Hubzone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: Y
Principal Investigator
 Theresa Curtis
 Staff Scientist
 (607) 272-0002
 tcurtis@agavebio.com
Business Contact
 Noe Salazar
Title: President
Phone: (512) 671-1369
Email: nsalazar@agavebio.com
Research Institution
N/A
Abstract
A PCR based assay has been developed to detect Leishmania parasites, but PCR requires sophisticated laboratory instruments and trained personnel to interpret the data. The parasite can also be identified in infected sand flies by skillful dissection and microscopic examination but this is subjective and not appropriate for field testing. Antibodies are available that can detect Leishmania parasites and a standard ELISA could be used for detection, but this like the other assays requires trained personnel and sophisticated laboratory instruments. To overcome these shortcomings, Agave BioSystems proposes to develop a dipstick assay to provide a practical tool for assessment of Leishmania infected sand flies in the field. Our novel dipstick assay would use new fluorescent quantum dot technology for detection. This quantum dot assay would be considerably more specific, sensitive, and stable making it superior to standard colorimetric dipstick assays.

* information listed above is at the time of submission.

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