Multiplex Aptamer-Based Sensor for Mosquito-borne Viruses

Award Information
Agency:
Department of Defense
Branch
Office of the Secretary of Defense
Amount:
$99,994.00
Award Year:
2010
Program:
SBIR
Phase:
Phase I
Contract:
W81XWH-10-C-0158
Award Id:
96883
Agency Tracking Number:
O093-H20-3181
Solicitation Year:
n/a
Solicitation Topic Code:
OSD 09-H20
Solicitation Number:
n/a
Small Business Information
4100 N. W. Loop 410, Suite 230, San Antonio, TX, 78229
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
154088710
Principal Investigator:
John Bruno
Senior Scientist
(210) 731-0015
john.bruno@otcorp.com
Business Contact:
William Henderson
Chief Financial Officer
(210) 731-0000
bhenders@otcorp.com
Research Institution:
n/a
Abstract
Operational Technologies Corporation (OpTech) has patents pending for: 1) one-step plastic-adherent aptamer-magnetic bead (MB) plus aptamer-quantum dot (QD) sandwich assays (that have already been successfully applied to ultrasensitive detection of Crimean-Congo Hemorrhagic Fever under a recent SBIR project), and 2) a multiplex cartridge in which the assays self-assemble as they traverse fluidic channels and adhere to plastic windows. Various sized QDs are utilized with a monochrome light source to enable simultaneous multicolored emissions for each type of captured pathogen present in a sample. OpTech originally developed this concept for rapid (< 15 minute) food and water safety testing, but can apply it to mosquito-borne virus detection and other clinical diagnostics as well in panels of 20 or more analytes. In Phase I, OpTech will develop DNA aptamers that distinguish Chikungunya, Dengue, and West Nile viruses and team with the Southwest Research Institute (SwRI) to develop a cartridge and portable fluorometer design for Phase II development. OpTech will attempt to demonstrate multiplex assay potential in Phase I by use of a spectrofluorometer and commercially available handheld fluorometer. OpTech will also deliver 100 prototype assays to the DoD at the conclusion of Phase I for independent testing and evaluation by the military.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government