Multiplex Aptamer-Based Sensor for Tick-borne Diseases

Award Information
Agency: Department of Defense
Branch: Army
Contract: W81XWH-10-C-0032
Agency Tracking Number: A093-156-0765
Amount: $69,985.00
Phase: Phase I
Program: SBIR
Awards Year: 2010
Solicitation Year: 2009
Solicitation Topic Code: A09-156
Solicitation Number: 2009.3
Small Business Information
4100 N. W. Loop 410, Suite 230, San Antonio, TX, 78229
DUNS: 154088710
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 John Bruno
 Senior Scientist
 (210) 731-0000
Business Contact
 William Henderson
Title: Chief Financial Officer
Phone: (210) 731-0000
Research Institution
Operational Technologies Corporation (OpTech) has patents pending for: 1) one-step plastic-adherent aptamer-magnetic bead (MB) plus aptamer-quantum dot (QD) sandwich assays (already successfully applied to ultrasensitive detection of Crimean-Congo Hemorrhagic Fever or CCHF under a recent Army SBIR project), and 2) a multiplex cartridge in which the assays self-assemble as they traverse microfluidic channels (< 100 µL). Various sized QDs can be utilized with a monochrome light source to enable simultaneous multicolored emissions for each type of captured pathogen in a clinical sample. OpTech developed this concept for rapid (< 15 minute) food and water safety testing, but can apply it to tickborne-borne viral and rickettsial detection in infected ticks or clinical samples in panels of 20 or more analytes. In Phase I, OpTech will develop DNA aptamers that distinguish Tick-Borne Encephalitis Virus (TBEV) from a spotted fever group rickettsia, and CCHF (using OpTech’s existing aptamers). OpTech will also team with Nanohmics, Inc., to develop a cartridge and portable fluorometer design for Phase II development. OpTech will attempt to demonstrate multiplex assay potential in Phase I by spectrofluorometry and deliver 100 prototype assays to the DoD at the conclusion of Phase I for independent testing and evaluation by the Army.

* Information listed above is at the time of submission. *

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government