Handheld Aptamer-Magnetic Bead-Quantum Dot Assays for Crimean-Congo Fever

Award Information
Agency:
Department of Defense
Branch
Army
Amount:
$729,989.00
Award Year:
2010
Program:
SBIR
Phase:
Phase II
Contract:
W81XWH-09-C-0029
Award Id:
92033
Agency Tracking Number:
A083-181-0021
Solicitation Year:
n/a
Solicitation Topic Code:
Army 08-181
Solicitation Number:
n/a
Small Business Information
4100 N. W. Loop 410, Suite 230, San Antonio, TX, 78229
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
154088710
Principal Investigator:
John Bruno
Senior Scientist
(210) 731-0000
john.bruno@otcorp.com
Business Contact:
William Henderson
Chief Financial Officer
(210) 731-0000
bhenders@otcorp.com
Research Institution:
n/a
Abstract
Operational Technologies Corporation (OpTech) has completed a successful Phase I proof-of-concept for a plastic-adherent DNA aptamer-magnetic bead (MB) plus aptamer-fluorescent nanoparticle assay for Crimean-Congo Hemorrhagic Fever (CCHF) virus in serum. By partitioning the assay away from the bulk solution, the signal-to-noise ratio increases greatly. Assays have been lyophilized and rehydrated with little or no loss of performance against key surface peptides, an albumin-peptide conjugate and irradiated CCHF virus. Therefore, OpTech proposes to continue development by testing the assay in serum with viable CCHF virus of several types and other related and unrelated viruses in BSL-4 laboratory facilities of the Southwest Research Foundation for Biomedical Research (SwFBR) co-located with OpTech in San Antonio. OpTech will conduct several rounds of live virus testing with the SwFBR and, if necessary, re-select aptamer sequences to detect the broadest possible array of CCHF viruses with ultrasensitivity. OpTechf?Ts commercial off-the-shelf handheld and battery-operated fluorometer will also be refitted with a new LED excitation source, red band pass emission filter, power adaptor for military vehicles, coupler to a PDA or small laptop, and more user friendly firmware. All assay engineering changes, protocols and formulations will be documented in detail in order to seek FDA approval.

* information listed above is at the time of submission.

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